FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325588
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAAACCCTAACAAAACCAAACGTTGGGGCTACTCTCTTAACTTTAT13920.42753418430654694No Hit
CATATAAACCCTAACAAAACCAAACGTTGGGGCTACTCTCTTAACTTTAT12850.39467056525424765No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT12040.36979249849503054No Hit
GACTTCGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAACAGTG10720.32925046377630623No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA10390.3191149550966252No Hit
CAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGG10300.31635072545671217No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10290.3160435888300552No Hit
TTTTATGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT10080.3095937196702581No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCG9820.30160816737717605No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCATCCTTTCCTCAC9760.29976534761723406No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9250.2841013796577269No Hit
GGTTTATATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAA8890.27304446109807484No Hit
GAGTAAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTA8250.2533877169920267No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT8000.2457093013256017No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7850.24110225192574666No Hit
GGTTCCTTGAGCAGGTGTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAG7830.24048797867243263No Hit
CCCACAAAATGAGGCGCTGCGTGTAGTTTCTCTCTTATATAGAATACCAG7640.23465238276594963No Hit
CATCCAGCGATAGCCAGGACAAGTTGGAGGACAAGAGGTTGGTGAGTGAT7400.22728110372618157No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7180.2205240979397275No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG7090.21775986829981447No Hit
CATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTC6880.21130999914001744No Hit
ATATAAGACCTTGGGCAATACCTGGCGGGCGTTCACGGTGGTCGCCATGC6790.20854576950010442No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCG6630.2036315834735924No Hit
GGTTCCTTGAGCAGGTGTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTGG6620.2033244468469354No Hit
TTCGTAAACTGCCTGTAAATAGACCTGTTGATTGGAAAGTATGTCAAAGA6510.19994594395370838No Hit
GGTGTTTTGAGCAGGTGTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAG6390.19626030443382433No Hit
CGCTTGATGTTTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCT6360.19533889455385334No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA6310.19380321142056833No Hit
CTTATATAGAATACCAGCCTGCCACAGAGTATGTAAATAATGTCTAGTTT6160.1891961620207133No Hit
CAAAACACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGG6090.18704620563411428No Hit
AAGGTATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGG5710.1753750138211482No Hit
GACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAACAGTG5410.16616091502143815No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCGTTAAC5190.1594039092349841No Hit
GTTGTCAATATGCCCTGAGCCTGCGGGCTCCACCCCAAAAGACTGCCGTG5110.15694681622172807No Hit
CGTGTGTGCTCCCCCTGGAAAATTGAGAGAAGTCCACCACGAGTCTAGAC5010.15387544995515806No Hit
TTTCCAGGGGGAGCACACACGTGGCCTGGCCAAAATTTGCAGTCCCCAAC4930.15141835694190203No Hit
GTTTTCGAAAACATTGCTTGATTTTTAGTACAATGTGTTCCTGTGGCAAT4910.15080408368858803No Hit
GCATCAATGCGGGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAGA4840.14865412730198901No Hit
GTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCT4780.146811307542047No Hit
CTCTTATATAGAATACCAGCCTGCCACAGAGTATGTAAATAATGTCTAGT4750.145889897662076No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC4670.14343280464882No Hit
CCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTGTGGTATTG4660.14312566802216298No Hit
TCTTCGTCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGG4660.14312566802216298No Hit
CTCTTAACTTTATGGGCTATGTAATTGGGAGTTGGGGTACATTGCCACAG4650.142818531395506No Hit
TTTTGTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT4610.14158998488887797No Hit
GTTTATATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4610.14158998488887797No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4530.13913289187562197No Hit
GACCAGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC4440.13636866223570893No Hit
ATGTTAATGTTAATATGGGCCTGAAACTCAGACAAATATTGTGGTTTCAC4440.13636866223570893No Hit
GTATACATGCATACAAAGGCATTAATGCGGGATAACCACATTGTGTAAAA4380.1345258424757669No Hit
GTCCCGTGCTGGTAGTTGAGGTTCCTGGAAGTAGAGGACAAACGGGCAAC4290.13176161283585391No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTTCACCAAGCTCTGCTAG4270.1311473395825399No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4250.1305330663292259No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC4180.12838310994262686No Hit
GAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTA4150.12746170006265586No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA4140.12715456343599885No Hit
GCCACAGAGTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCA4120.12654029018268487No Hit
GTCAGGAAGACAGCCTACTCCCATTTCTCCACCTCTAAGAGACAGTCATC4040.12408319716942885No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4020.12346892391611486No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4000.12285465066280085No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3920.12039755764954484No Hit
TTCGAAAACTGCCTGTAAATAGACCTGTTGATTGGAAAGTATGTCAAAGA3910.12009042102288782No Hit
GCCCAGTGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC3890.11947614776957381No Hit
CTTTAATCCTGAGTGGCAAACTCCATCCTTTCCTCACATTCATTTGAAGG3880.11916901114291682No Hit
GCACCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTC3870.1188618745162598No Hit
ATGTTTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGCCT3780.1160976448763468No Hit
AAGCCATTCTGTGTTGGGGTGAGTTGATGAATCTGGCAACCTGGGTGGGA3720.11425482511640478No Hit
CTCCAGAACATTGTTCACCTCACCATACAGCACTCAGGCAAGCTATTCTG3710.11394768848974778No Hit
CTCTGTGGCAGGCTGGTATTCTATATAAGAGAGAAACTACACGCAGCGCC3700.11364055186309079No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3690.11333341523643377No Hit
CCCAAGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC3680.11302627860977678No Hit
ATTTACATACTCTGTGGCAGGCTGGTATTCTATATAAGAGAGAAACTACA3660.11241200535646277No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3640.11179773210314876No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT3600.11056918559652076No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3590.11026204896986375No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA3550.10903350246323573No Hit
CCCTAGAAAACTGAGAGAAGTCCACCACGAGTCTAGACTCTGTGGTATTG3530.10841922920992172No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG3530.10841922920992172No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3520.10811209258326474No Hit
AAACTGTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCT3520.10811209258326474No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3480.10688354607663673No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3470.10657640944997974No Hit
GTTCCGGAGACTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGG3410.10473358969003772No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC3360.1031979065567527No Hit
GGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACCAACCTCTTGTCC3350.10289076993009572No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT3350.10289076993009572No Hit
CCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCC3340.1025836333034387No Hit
GCCTAGTGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC3340.1025836333034387No Hit
CAATATGTGGGCCCCCTCACAGTTAACGAAAAAAGGAGATTAAAATTAAT3260.10012654029018268No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3260.10012654029018268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)