FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369972
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17390.470035570259371No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC14500.3919215508200621No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA14390.3889483528483237No Hit
TCCCTAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCG13770.3721903279167072No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGATCACCATATT13010.35164823283924185No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12250.33110613776177655No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11480.31029375195960773No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10800.29191398267977037No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10350.27975090006811326No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT9930.2683986896305667No Hit
GTGTAAAAGGGGCGGCAAAACCCAGAAGACCCACAATACGTTGACAGACT9570.25866822354124097No Hit
ATATCAGAGAGAAACAACACATAGCGCCTCATTTTGTGGATCACCATATT9130.24677543165428734No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8920.24109932643551404No Hit
ATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAAC8810.23812612846377565No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC8610.2327203139697058No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA7750.20947531164520558No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7640.20650211367346719No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7420.20055571772999037No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7000.18920350729244376No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCGTC6810.18406798352307743No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6740.18217594845015297No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6430.17379693598434476No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6390.1727157730855308No Hit
CACCTGGCCAGACGCCAACAAGGTAGGAGCTGGAGCATTCGGGCTGGGAT6280.1697425751137924No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT6160.1664990864173505No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6060.1637961791703156No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6030.16298530699620511No Hit
GAGGAACGGTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACT6020.16271501627150164No Hit
GAGATACGGTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACT5760.15568745742921086No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCCGTG5740.15514687597980387No Hit
CTCTATGACCGTTCCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5720.1546062945303969No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5580.15082222438454804No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCCTCGGACGGAAATTGCACC5490.1483896078622166No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5400.14595699133988518No Hit
GTTTAATACCCTTGTCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC5380.1454164098904782No Hit
CCGTTCCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAATAGTTGC5370.1451461191657747No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5290.1429837933681468No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5240.14163233974462933No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5200.14055117684581536No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTTCACATTTCTT5170.1397403046717049No Hit
CTCATGAGCATTGTTCACCTCACCATACGGCACTCAGGCAAGCAATTCTT5110.13811856032348394No Hit
GTTGACGATCCTGGAATTAGAGGACAAACGGGCAACATACCTTGATAGTC5040.1362265252505595No Hit
GGCCCCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTC5040.1362265252505595No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5030.135956234525856No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC5030.135956234525856No Hit
CATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCGGCA4990.13487507162704204No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4950.13379390872822808No Hit
ATCTTCAACCACCAGCACGGGACCCTGCAGAACCTGCACGACTCCTGCTC4950.13379390872822808No Hit
ACTAAATGTTCTGGATAATAAGGTTTAATACCCTTGTCCAATGGCAAATA4900.13244245510471062No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT4890.13217216438000712No Hit
CCGTATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAATAGTTGC4830.13055042003178619No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTCGACAACGGCCAGGTC4810.13000983858237922No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4720.1275772220600478No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4610.12460402408830938No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT4600.12433373336360588No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4570.12352286118949543No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGATCACCATA4540.12271198901538495No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4530.12244169829068147No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT4510.12190111684127448No Hit
GTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAA4410.11919820959423956No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG4390.11865762814483258No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4360.1178467559707221No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCC4350.11757646524601861No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC4320.11676559307190815No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4320.11676559307190815No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4240.11460326727428022No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4210.11379239510016974No Hit
AGTCGGAGGACAGGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAAT4190.11325181365076276No Hit
ATCCAACATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC4120.11135977857783831No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG4070.11000832495432088No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4040.1091974527802104No Hit
GCTTTGGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG4000.10811628988139643No Hit
GGATAACCACATTGTGTAAAAGGGGCGGCAAAACCCAGAAGACCCACAAT3960.10703512698258247No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGTTGATGGCC3910.105683673359065No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3890.10514309190965802No Hit
CTTTGGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA3880.10487280118495453No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA3880.10487280118495453No Hit
CCTTTACCCCGTTGCTCGACAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3830.10352134756143709No Hit
TGCCTAAACTTTAGGCCCATATTAGTGTTAACATAACTGACTACTAGGTC3750.10135902176380915No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT3730.10081844031440217No Hit
GTTTGGTGGAAGGTTGTGGAGTTCCACTGCATGGCCTGAGGATGAGTGTT3710.10027785886499518No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)