FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520296
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC24620.47319218291126586No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTTGAAGTAATGATTAACTAGA17620.3386533819210603No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG17330.3330796315943232No Hit
CATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTC17300.33250303673293663No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATCTTTTGTACGATGTGTT16190.3111690268616326No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGATACTCCCTAAATTTTAT16050.3084782508418285No Hit
GTGCTGGTGCGCGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14720.28291587865368945No Hit
CATATAAACCCTAACAAAACAAAGAGATGGGGATACTCCCTAAATTTTAT14480.2783031197625967No Hit
CTCTATAACTGTATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA13820.26561803281209156No Hit
CTATGACTCCCTCCTGTTGCTGTACCAAACCTTCGGCCGGAAATTGCACC13760.2644648430893184No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13390.2573535064655504No Hit
TCCTCAGGCCATGCAGTGGAACAACACAACCTTCCACCAAACTGCTGGTG12950.24889678183188033No Hit
CCTCCAGCATATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA12330.2369804880298907No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA12220.23486630687147317No Hit
CTCCCGCTCCTACCTTGTTGGCGTCTGGCCAGGTGTCCTTGTTGGGATTG12160.23371311714869997No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC12000.23063794455463812No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT11980.2302535479803804No Hit
CCCTTCTCCAATGGTAAATATTTTGTAACCTTTGGATAAAACCTAGCAGG11840.22756277196057628No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT11380.2187216507526485No Hit
CCTATTAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT11180.21487768501007118No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC11100.21334009871304027No Hit
CCCATGATGATGGGATGGGAATACAGGTGCAATTTCCGGCCGAAGGTTTG11030.21199471070313822No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA10680.20526777065362792No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGATCTTTTGTACGATGTGTT10490.20161600319817952No Hit
GGGTCTCACCCCACCGCACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTC10330.19854083060411765No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT9810.18854651967341668No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT9690.18624014022787028No Hit
ATGTCATTGGAAGTTATGGGTCCTTGCCACAAGAACACATCGTACAAAAG9550.18354936420806617No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC9370.18008979503974662No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC9250.17778341559420022No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA9160.17605363101004043No Hit
GAATATTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA9000.1729784584159786No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT8930.17163307040607656No Hit
GCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCC8890.17086427725756106No Hit
GGTTTATATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA8630.16586712179221058No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8310.1597167766040869No Hit
TGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA8300.15952457831695804No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC8280.1591401817427003No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8210.15779479373279826No Hit
CCTTATGAGTCCAAGGAATATTAACATTGAGATCCCCGAGATTGAGATCT8150.15664160401002505No Hit
GATCCAGCATCCAGAGACCTAGTAGTCAGTTATGTCAACACTAATATGGG8140.1564494057228962No Hit
CTACAAACTTTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC8080.155296216000123No Hit
GCGCGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCT8040.15452742285160756No Hit
GCCTGAGGATGAGTATTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT7900.15183664683180342No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7830.15049125882190137No Hit
GTGTGAAAGGTGCAGCAAAACCCAAAAGACCCACAATTCGTTGACATACT7710.148184879376355No Hit
GGATTCATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCGGCATGGTCC7570.14549410335655089No Hit
CAATAACATATCCCATAAAATTTAGGGAGTATCCCCATCTCTTTGTTTTG7530.14472531020803542No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC7510.14434091363377768No Hit
CTATAACTGTATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG7370.14165013761397358No Hit
GCTCCTGAGACTCTAAGGCTTCCCGAAACAGAGCTGAGGCGGTATCTAGA7330.14088134446545814No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7330.14088134446545814No Hit
CTATGAATCCCTCCTGTTGCTGTACCAAACCTTCGGCCGGAAATTGCACC7320.14068914617832926No Hit
CATATAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAAGGTGCAGCA7300.14030474960407155No Hit
GTGTAAATAGTGTCTAGTTTTGAAGTAATGATTAACTAGATGTTCTGGAT7300.14030474960407155No Hit
TCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT7200.13838276673278288No Hit
AATTTAGGGAGTATCCCCATCTCTTTGTTTTGTTAGGGTTTAAATGTATG7090.13626858557436536No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT6980.13415440441594784No Hit
CCCTTATCCAATGGTAAATATTTTGTAACCTTTGGATAAAACCTAGCAGG6900.13261681811891693No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6820.131079231821886No Hit
GTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6790.1305026369604994No Hit
CTCCAGCTCCTACCTTGTTGGCGTCTGGCCAGGTGTCCTTGTTGGGATTG6680.12838845580208189No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGTTTGGTGGAAGGTTGT6650.1278118609406953No Hit
ACATCGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTT6540.12569767978227778No Hit
GGATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCGGCATGATCC6490.12473668834663346No Hit
CCCTAAATTTTATGGGATATGTCATTGGAAGTTATGGGTCCTTGCCACAA6460.12416009348524687No Hit
GGATAGGGGCATTTGGTGGTCTATATGCTGGAGGAGTGCGAATCCACACT6390.1228147054753448No Hit
TACCCTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6380.12262250718821593No Hit
TCCTATTAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTC6370.12243030890108707No Hit
GACATCGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTT6350.12204591232682935No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG6330.12166151575257161No Hit
GAATACATGCATATAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAA6230.11973953288128296No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6220.11954733459415409No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGGAACTTTACTGGGCTTTATTC6070.11666436028722112No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6040.11608776542583453No Hit
CCTTTCACACAATGTGGTTATCCTGCTTTAATGCCCTTATATGCATGTAT6020.11570336885157678No Hit
GATCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCT5880.11301259283177267No Hit
GAGCTGGAGCATTCGGGCTGGGTTTCACCCCACCGCACGGAGGCCTTTTG5860.11262819625751497No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCTTATCGTCAATCTTCTCG5830.11205160139612835No Hit
TCCTAGTACAAAGACCTTTAATCTAAGCTCCTCCCCCAACTCCTCCCAGT5820.11185940310899949No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5810.11166720482187063No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGCATACATTTAAA5770.11089841167335515No Hit
CTCAGGAGCATTGTTCACCTCACCATACTGCACTCAGGCAAGCAACTCTT5710.10974522195058198No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTT5710.10974522195058198No Hit
GCTCAGAAGACTCTAAGGCTTCCCGAAACAGAGCTGAGGCGGTATCTAGA5710.10974522195058198No Hit
CATTTACACCAAGATATTATCAAAAAATGTGAACAATTTGTAGGCCCACT5710.10974522195058198No Hit
ATGTATATTTGCGGGAGAGGACAACAGAGTTATCAGTCCCGATAATGTTT5610.10782323907929334No Hit
GTGCTGGTGCGCAGACCATTTTATGCCTACAGCCTCCTAGTACAAAGACC5610.10782323907929334No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGATTAAAGA5550.10667004935652014No Hit
GTATAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC5490.10551685963374693No Hit
TCACCCCACCGCACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAGGGC5460.10494026477236035No Hit
TAACCACATTGTGTGAAAGGTGCAGCAAAACCCAAAAGACCCACAATTCG5430.10436366991097375No Hit
GTCTTCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCAAGCAACTC5410.10397927333671603No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5390.1035948767624583No Hit
GGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGC5380.10340267847532943No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5370.10321048018820057No Hit
CTTCTGGACTATCAGGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5350.10282608361394284No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT5340.10263388532681396No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5310.10205729046542737No Hit
GCTCAGTAGACTCTAAGGCTTCCCGAAACAGAGCTGAGGCGGTATCTAGA5250.10090410074265418No Hit
CCCTACATTTTATGGGATATGTCATTGGAAGTTATGGGTCCTTGCCACAA5220.1003275058812676No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTCCCCACCTTATGAGTCCAAGGAAT5220.1003275058812676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)