FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520296
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC21970.42225963682211665No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTTGAAGTAATGATTAACTAGA17430.3350016144656119No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG16910.3250073035349109No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATCTTTTGTACGATGTGTT16530.31770376862401406No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGATACTCCCTAAATTTTAT15980.3071328628319265No Hit
CATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTC15950.3065562679705398No Hit
CTCTATAACTGTATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA14180.2725371711487307No Hit
CATATAAACCCTAACAAAACAAAGAGATGGGGATACTCCCTAAATTTTAT13740.26408044651506063No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13630.26196626535664314No Hit
GTGCTGGTGCGCGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12210.2346741085843443No Hit
CTATGACTCCCTCCTGTTGCTGTACCAAACCTTCGGCCGGAAATTGCACC12030.2312145394160247No Hit
CCTCCAGCATATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA12010.23083014284176698No Hit
TCCTCAGGCCATGCAGTGGAACAACACAACCTTCCACCAAACTGCTGGTG11920.22910035825760722No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT11830.22737057367344743No Hit
CCCTTCTCCAATGGTAAATATTTTGTAACCTTTGGATAAAACCTAGCAGG11510.22122022848532372No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC11430.2196826421882928No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA11330.21776065931700417No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT11100.21334009871304027No Hit
CCTATTAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT10150.19508126143579807No Hit
CTCCCGCTCCTACCTTGTTGGCGTCTGGCCAGGTGTCCTTGTTGGGATTG10000.1921982871288651No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGATCTTTTGTACGATGTGTT9930.19085289911896305No Hit
GGGTCTCACCCCACCGCACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTC9600.1845103556437105No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA9360.17989759675261774No Hit
ATGTCATTGGAAGTTATGGGTCCTTGCCACAAGAACACATCGTACAAAAG9130.17547703614865384No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT8910.1712486738318188No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC8890.17086427725756106No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT8750.16817350123775696No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8700.16721250980211264No Hit
CCCATGATGATGGGATGGGAATACAGGTGCAATTTCCGGCCGAAGGTTTG8670.16663591494072605No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA8500.16336854405953533No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC8350.16048556975260236No Hit
GCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCC8350.16048556975260236No Hit
GAATATTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA8070.15510401771299415No Hit
CTATAACTGTATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG8030.15433522456447868No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC8020.15414302627734983No Hit
TGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA7980.15337423312883436No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7960.15298983655457662No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT7940.1526054399803189No Hit
GTGTGAAAGGTGCAGCAAAACCCAAAAGACCCACAATTCGTTGACATACT7930.15241324169319004No Hit
GTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7830.15049125882190137No Hit
GCCTGAGGATGAGTATTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT7690.1478004828020973No Hit
GGTTTATATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA7690.1478004828020973No Hit
CCTTATGAGTCCAAGGAATATTAACATTGAGATCCCCGAGATTGAGATCT7660.14722388794071067No Hit
CTACAAACTTTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC7610.14626289650506635No Hit
CATATAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAAGGTGCAGCA7460.14337992219813336No Hit
GATCCAGCATCCAGAGACCTAGTAGTCAGTTATGTCAACACTAATATGGG7400.1422267324753602No Hit
CCCTTATCCAATGGTAAATATTTTGTAACCTTTGGATAAAACCTAGCAGG7320.14068914617832926No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCTTATCGTCAATCTTCTCG7260.13953595645555605No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC7230.13895936159416947No Hit
GTGTAAATAGTGTCTAGTTTTGAAGTAATGATTAACTAGATGTTCTGGAT7220.13876716330704061No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT7090.13626858557436536No Hit
CTATGAATCCCTCCTGTTGCTGTACCAAACCTTCGGCCGGAAATTGCACC7040.13530759413872104No Hit
CAATAACATATCCCATAAAATTTAGGGAGTATCCCCATCTCTTTGTTTTG6920.13300121469317466No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC6900.13261681811891693No Hit
AATTTAGGGAGTATCCCCATCTCTTTGTTTTGTTAGGGTTTAAATGTATG6870.13204022325753031No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6780.13031043867337055No Hit
GGATTCATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCGGCATGGTCC6760.1299260420991128No Hit
GACATCGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTT6670.12819625751495303No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6660.12800405922782415No Hit
GTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA6400.12300690376247367No Hit
GGATAGGGGCATTTGGTGGTCTATATGCTGGAGGAGTGCGAATCCACACT6390.1228147054753448No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTT6390.1228147054753448No Hit
CTCCAGCTCCTACCTTGTTGGCGTCTGGCCAGGTGTCCTTGTTGGGATTG6230.11973953288128296No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6170.11858634315850976No Hit
TCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT6170.11858634315850976No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6150.11820194658425205No Hit
CATTTACACCAAGATATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6150.11820194658425205No Hit
CCCTAAATTTTATGGGATATGTCATTGGAAGTTATGGGTCCTTGCCACAA6130.1178175500099943No Hit
TCCTATTAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTC6120.11762535172286544No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6120.11762535172286544No Hit
ACATCGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTT6080.11685655857434998No Hit
GAATACATGCATATAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAA6000.11531897227731906No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGGAACTTTACTGGGCTTTATTC5910.11358918769315929No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGTTTGGTGGAAGGTTGT5850.11243599797038609No Hit
GCGCGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCT5830.11205160139612835No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGATTAAAGA5770.11089841167335515No Hit
GCACGGGACCATGCCGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5710.10974522195058198No Hit
GCTCCTGAGACTCTAAGGCTTCCCGAAACAGAGCTGAGGCGGTATCTAGA5690.10936082537632424No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5690.10936082537632424No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG5660.10878423051493766No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG5640.10839983394067992No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5570.10705444593077787No Hit
GATCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCT5550.10667004935652014No Hit
ATGTTATTGGAAGTTATGGGTCCTTGCCACAAGAACACATCGTACAAAAG5530.1062856527822624No Hit
CTTCTGGACTATCAGGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5490.10551685963374693No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTCCCCACCTTATGAGTCCAAGGAAT5480.10532466134661807No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5470.10513246305948923No Hit
TACCCTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5470.10513246305948923No Hit
GGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGC5350.10282608361394284No Hit
CCTTTCACACAATGTGGTTATCCTGCTTTAATGCCCTTATATGCATGTAT5350.10282608361394284No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGCATACATTTAAA5310.10205729046542737No Hit
GGATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCGGCATGATCC5290.10167289389116964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)