FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448263
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC16160.360502651345304No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14790.32994023597753996No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13800.30785498691616303No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12580.2806388214061834No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC12500.2788541548153651No Hit
GTATACATGCATATAAAGGCATCAAGGCGGGATAACCACATTGTGTAAAA12360.275730988281433No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC12360.275730988281433No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11650.2598920722879203No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA11400.254314989191613No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10840.2418223230558846No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC10770.24026073978891857No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10710.23892223984580482No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10050.22419874047155353No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG9700.2163908241367233No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8960.19988265817165368No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8750.19519790837075557No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC8740.19497482504690328No Hit
TCCACAGAGTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8710.1943055750753464No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8270.18448990882584554No Hit
CCTTTTACACAATGTGGTTATCCCGCCTTGATGCCTTTATATGCATGTAT8250.18404374217814096No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8160.18203599226347034No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8070.1800282423487997No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7940.1771281591387199No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC7830.1746742425763447No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7700.1717741593662649No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7560.1686509928323328No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7480.16686632624151446No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7440.1659739929461053No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACGCAGCGCT7430.165750909622253No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7260.16195849311676405No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7220.1610661598213549No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7200.16061999317365028No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7170.15995074320209343No Hit
GACCAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTG7040.15705065999201362No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAATGAGGGAGTTTG6960.15526599340119526No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAAT6930.15459674342963842No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6860.15303516016267235No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6810.1519197435434109No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6780.15125049357185402No Hit
CCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGT6760.15080432692414944No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC6690.1492427436571834No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG6690.1492427436571834No Hit
GTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG6680.1490196603333311No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT6270.13987324405538715No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACCGAGTTGTCAGTC6210.13853474411227337No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC6160.13741932749301192No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6010.13407307763522752No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6000.13384999431137523No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5880.13117299442514774No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5750.12827291121506795No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5740.12804982789121566No Hit
ACCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG5660.1262651613003973No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5610.12514974468113585No Hit
ACTCACCCCAACACAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAA5610.12514974468113585No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5610.12514974468113585No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5540.12358816141416981No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5460.12180349482335147No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5400.12046499488023771No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC5350.11934957826097625No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5350.11934957826097625No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG5330.11890341161327168No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5320.11868032828941939No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5320.11868032828941939No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA5210.11622641172704418No Hit
CATATAAAGGCATCAAGGCGGGATAACCACATTGTGTAAAAGGAGCAGCA5200.11600332840319189No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA5200.11600332840319189No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5190.11578024507933958No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT5120.11421866181237354No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5120.11421866181237354No Hit
ATATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG5110.11399557848852124No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4990.11131857860229376No Hit
ATTTACACACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA4940.11020316198303227No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4940.11020316198303227No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4920.1097569953353277No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4900.10931082868762311No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4900.10931082868762311No Hit
ATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC4870.10864157871606625No Hit
CTTCATCTGCCGTTCCAGCCGACCACGGGGCGCACCTCTCTTTACGCGGT4870.10864157871606625No Hit
AGGTTCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGT4850.10819541206836165No Hit
ACCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG4750.10596457882983874No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4680.10440299556287269No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4620.10306449561975893No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4600.10261832897205435No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG4570.10194907900049748No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4550.10150291235279289No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCT4530.1010567457050883No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4530.1010567457050883No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA4520.10083366238123602No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4500.10038749573353144No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)