FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332146
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15810.4759954959565974No Hit
GTTAATATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC9860.29685740608045863No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT6550.19720243507373264No Hit
GAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT6100.18365417617553725No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA6100.18365417617553725No Hit
TGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATG5880.17703058293641952No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT5870.17672951051645963No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA5760.17341771389690078No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5550.16709519307774293No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA5330.1604715998386252No Hit
GAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT5310.1598694549987054No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC5210.15685873079910642No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5060.15234264449970797No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5060.15234264449970797No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG4930.14842870304022932No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC4600.1384933131815527No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4550.1369879510817532No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT4460.13427829930211413No Hit
CCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA4410.13277293720231464No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4350.13096650268255527No Hit
AATTTGAGGACAACAGGTTGGTGAGTGACTGGAGATTTGGGACTGCGAAT4290.12916006816279588No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA4260.1282568509029162No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4210.1267514888031167No Hit
CCTTTCCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA4130.12434290944343751No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA4090.12313861976359793No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCAGGCC3990.12012789556399897No Hit
CATATATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3890.11711717136439999No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA3790.11410644716480102No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA3680.11079465054524215No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG3660.11019250570532237No Hit
TTTTACGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTT3600.10838607118556298No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA3530.1062785642458437No Hit
ATTGTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA3510.10567641940592389No Hit
ATATAAATATCCTGCTTTAATGCCTTTATATGCATGTATAC3400.10236462278636503No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3390.10206355036640515No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC3390.10206355036640515No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)