FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332146
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15730.4735869165969182No Hit
GTTAATATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC9050.2724705400637069No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA6540.19690136265377275No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT6100.18365417617553725No Hit
GAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT5360.16137481709850487No Hit
TGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATG5080.15294478933962774No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC4770.14361154432087095No Hit
GAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT4690.14120296496119175No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC4580.1378911683416329No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT4470.13457937172207404No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4350.13096650268255527No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4230.12735363364303648No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC4160.12524612670331722No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA4140.12464398186339742No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA4010.12073004040391877No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA3820.11500966442468072No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT3610.10868714360552287No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGT3530.1062785642458437No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA3440.10356891246620462No Hit
AATTTGAGGACAACAGGTTGGTGAGTGACTGGAGATTTGGGACTGCGAAT3370.10146140552648535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)