FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435137
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30050.6905871024527908No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTCACAGTTAACG18260.4196379531044246No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG16510.37942073415958655No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT12440.2858869735278774No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10780.24773806870020246No Hit
GTTCTATCCTAACCGTACAAAATATTTGCCCTTAGATAAAGGCATTAAAC9770.22452698805203877No Hit
ATCCTAACCGTACAAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAC9490.2180922330208647No Hit
ATGTAGGCCCTCTCACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG8730.20062646936482073No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGACCCACAATTCGTTGACATACT8650.1987879679273424No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC8300.1907445241383748No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT8030.1845395817868855No Hit
ACAATATGTAGGCCCTCTCACAGTTAACGAAAAAAGGAGATTAAAATTGA7820.17971351551350495No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7370.16937194492768942No Hit
GGATAGTAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTTGTACGGTT7280.16730363081052635No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT6700.15397449538880859No Hit
GTTCAGGATAGTAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTTGTA6640.15259561931069984No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG6620.1521359939513303No Hit
ATTAACAGGAAGTTTCCGAAAACATTGTTTAATTTTGTGTACGATATGTT6430.14776955303731928No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6410.1473099276779497No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC6380.14662048963889535No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6230.1431732994436235No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTCACAGTTAACGAAAAAAGGA6230.1431732994436235No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6230.1431732994436235No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6040.1388068585296125No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5960.1369683570921342No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5930.13627891905307982No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTCACAGTTAACGAAAAAA5850.13444041761560152No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC5680.1305336020609601No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5580.12823547526411222No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG5570.12800566258442744No Hit
GCACAGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAGCCTCTATG5550.12754603722505786No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG5360.12317959631104687No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA5340.12271997095167728No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGACC5120.11766409199861193No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG5120.11766409199861193No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5110.11743427931892715No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5100.11720446663924236No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA5090.11697465395955756No Hit
ATCTAAGGGCAAATATTTTGTACGGTTAGGATAGAACCTAGCAGGCATAA5080.11674484127987277No Hit
ATGTAAGACCTTGGGCAAGACCTGTTGGGCGTTCACGGTGGTCTCCATGC5040.1158255905611336No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGT5030.11559577788144884No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5010.11513615252207925No Hit
CTCTCACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG4980.11444671448302489No Hit
GATTAACTATGTGTTCAGGATAGTAAGGTTTAATGCCTTTATCTAAGGGC4920.11306783840491616No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4900.11260821304554657No Hit
GTCCTGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4890.1123784003658618No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG4890.1123784003658618No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4880.112148587686177No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGA4830.11099952428775305No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTCACAGTTAACGAAAA4670.10732252141279641No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG4670.10732252141279641No Hit
ATTTTGTACGGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTC4640.10663308337374205No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACC4620.10617345801437249No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG4600.10571383265500289No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4410.10134739174099193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)