FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492502
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25720.5222313818014952No Hit
CTTTTATATAAGATGCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT13520.27451665170902856No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT12510.25400912077514404No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA10800.21928844959005242No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA10110.20527835419957685No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9580.19451697658080577No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9460.19208043825202742No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACACCCGTG9400.19086216908763823No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9140.18558300270861844No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8890.1805068811903302No Hit
GTCTCTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA8790.1784764325830149No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8780.17827338772228335No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8770.17807034286155182No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8050.16345111288888167No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT7550.15329886985230518No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT7480.15187755582718446No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACGTTTCCT7180.14578621000523856No Hit
ATATTAACGTTGACATAGCTGACTACTGCTTCCCTGGATGCTGGATCTTC7140.14497403056231245No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA7100.14416185111938631No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6740.13685223613305125No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG6350.12893348656452158No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6220.12629390337501167No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6040.12263909588184413No Hit
CTCTTTTATATAAGATGCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC5960.1210147369959919No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5930.1204056024137973No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5860.11898428838867658No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT5620.11411121173111986No Hit
CTCTTTGGAAGGCGGGCATCTTATATAAAAGAGAGTCCACACGTAGCGCC5590.11350207714892527No Hit
TTCAAGAACAGTGTCTCTCCCAAAAGTAAGACAGGAAACGTGAAACCACA5550.11268989770599916No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5430.1102533593772208No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCAAGTATACAAGCAAAACAG5430.1102533593772208No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5420.11005031451648928No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA5400.10964422479502621No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT5330.1082229107699055No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5310.10781682104844244No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC5260.1068015967447848No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5250.10659855188405326No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5170.10497419299820103No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5080.10314678925161726No Hit
TCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGG5040.10233460980869112No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTGTCTCTCCCAAAAGTAAGA5000.10152243036576501No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4990.10131938550503349No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4980.10111634064430194No Hit
GGGTAAAGGTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGA4940.10030416120137584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)