FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451920
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG13630.3016020534607895No Hit
GTTAATATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC12720.281465746149761No Hit
TCTATAGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTGTTCG11530.25513365197380067No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC11510.2546910957691627No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGAAAGTAATGATTAACTACA11260.2491591432111878No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT10770.2383165161975571No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10160.22481855195609843No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA10160.22481855195609843No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTTCAGACGCGACATTATT9830.2175163745795716No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9160.202690741724199No Hit
CTTTTATATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT8800.19472473004071517No Hit
GAATACTAACATTGGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA8530.18875022127810231No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG8160.18056293149229952No Hit
CATTACCACAGGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAA7980.17657992565055763No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7940.17569481324128167No Hit
ATATGTTCCTGTGGTAATGTGCCCCAACTCCCAATTACATATCCCATGAA7740.17126925119490174No Hit
CTACTTGTGGTCTCTCTTTTATATAAGATTCCCGCCTTCCAAAGAGTGTG7720.17082669499026376No Hit
CCTTTATGCTGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7680.1699415825809878No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTAGTTGACATTGCTGAAAGTCCAA7540.16684368914852188No Hit
CCCTTAACTCCACCTCTAAGGGACACCCATCCTCAGGCCGTGCAGTGGAA7450.1648521862276509No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7410.16396707381837494No Hit
CCTTTTTTCATTTTTTGTAAGGGGCCCCACAAATTGCTCACATCGATCAA7350.16263940520446096No Hit
CGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCCT7290.161311736590547No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7280.161090458488228No Hit
GAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT6990.15467339352097717No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6970.1542308373163392No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6890.15246061249778722No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6880.15223933439546822No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6470.14316693220038945No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6400.14161798548415647No Hit
ATCCAGAGCATGTAGTTAATCATTACTTTCAGACGCGACATTATTTACAC6220.13763497964241458No Hit
CATTTAAATCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6140.13586475482386262No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACAGAAT6060.13409453000531069No Hit
ATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCT5980.13232430518675872No Hit
GAGGCAGATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAA5920.13099663657284477No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5870.12989024606124977No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5820.1287838555496548No Hit
ATTCTATGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAGGTAA5780.12789874314037883No Hit
GTGTAAATAATGTCGCGTCTGAAAGTAATGATTAACTACATGCTCTGGAT5580.12347318109399893No Hit
GTGGTAATGTGCCCCAACTCCCAATTACATATCCCATGAAGTTAAGGGAA5560.12303062488936095No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5520.12214551248008497No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5500.12170295627544699No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCATCACTTCCGGA5450.12059656576385201No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5420.11993273145689504No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG5360.11860506284298104No Hit
CTCTTTTATATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC5310.11749867233138608No Hit
ATTTTTCGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGGT5240.11594972561515313No Hit
GCATAAAGGGACTCAAGATGTTGTACAGATTTGGCCCCCAAAACCACATC5190.11484333510355815No Hit
ATCAACCATCCAGGGAATTAGTAGTCGGCTATGTCAACGTTAATATGGGC5180.11462205700123916No Hit
AAGTAAGACAGGAAATGTGAAACCACAATAGTTGTCTGAATTTTAGGCCC5160.11417950079660116No Hit
GTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCAAAACAG5160.11417950079660116No Hit
CCTTAATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5160.11417950079660116No Hit
CTACGTGTGGACTCTCTTTTATATAAGATTCCCGCCTTCCAAAGAGTGTG5150.11395822269428219No Hit
TCCTCAGGCCGTGCAGTGGAATTCCACCACTTTCCACCAAACTCTTCAAG5060.11196671977341123No Hit
ATATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGAA5060.11196671977341123No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTGTC5010.11086032926181624No Hit
GAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT5000.11063905115949726No Hit
TGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATG4990.11041777305717826No Hit
CTGTTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCACAACTGTGCCAGC4990.11041777305717826No Hit
GGGTAAAGGTTCAGATACTGGTTACGTAAAAAGGCCTTGTAAGTTGGCGA4910.10864754823862631No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC4870.10776243582935033No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4870.10776243582935033No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTT4840.10709860152239334No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGGAT4820.10665604531775534No Hit
GAATATTAACATTGGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4810.10643476721543636No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4800.10621348911311736No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC4800.10621348911311736No Hit
ATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAATCCT4780.10577093290847937No Hit
CCCATAGGGATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4740.10488582049920339No Hit
GCCACATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAACATGGA4720.10444326429456541No Hit
GTGTGTAAATAATGTCGCGTCTGAAAGTAATGATTAACTACATGCTCTGG4660.10311559568065144No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4630.10245176137369447No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4570.10112409275978049No Hit
CTTCTATAGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTGTT4570.10112409275978049No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT4550.10068153655514249No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4530.1002389803505045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)