FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419151
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCAGG13540.3230339424217048No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA11770.28080572395151154No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT11580.27627275134736645No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT10330.24645056316220168No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10200.24334905559094455No Hit
GTGTAAAGGGAGCGGCAAAGCCTAGAAGCCCCACAATTCGCTGACATACT9890.2359531529210237No Hit
CCTACAAATAGACCTATTGATTGGAAAGTATGTCAGCGAATTGTGGGGCT9270.22116134758118194No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAGCGAATTGTGGGGCT8700.20756242976874684No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8590.20493807720845233No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8360.19945079458238202No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAACTTTAACCTCCTTTTTTCG8040.19181631440697983No Hit
ATTTGTAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT6480.15459822355189418No Hit
CATGTATACAAGCAAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6480.15459822355189418No Hit
TCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA6460.15412106854093155No Hit
ATTCAGAGCCAATTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG6430.15340533602448758No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAA6390.15245102600256233No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAACG6370.1519738709915997No Hit
ATTTACATACTCTGTGGAAGGCCGGAATTTTATATAAGAGAGAAACTACT6360.15173529348611836No Hit
CTCTTACAATTAACGAAAAAAGGAGGTTAAAGTTAATTATGCCTGCTAGG6340.15125813847515573No Hit
CTACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTA6290.15006525094774914No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT6290.15006525094774914No Hit
CGGCAATCTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACAG6080.14505512333264145No Hit
GTTAGTATACCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5930.1414764607504217No Hit
TCCACAGAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATG5770.13765922066272057No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT5760.1374206431572393No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC5750.13718206565175797No Hit
CATTTAAACCCTACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5560.1326490930476129No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG5330.1271618104215426No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG5330.1271618104215426No Hit
AGACCGCCGTGTGGTGGAGTGAACCCTGGCCCGAAGGATCCCACTCCTAC5320.12692323291606128No Hit
TTTACATACTCTGTGGAAGGCCGGAATTTTATATAAGAGAGAAACTACTC5210.12429888035576676No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5100.12167452779547228No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4940.11785728770777118No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT4910.11714155519132724No Hit
GGATAGAACCTAGCAGGCATAATTAACTTTAACCTCCTTTTTTCGTTAAT4770.11380147011458878No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4770.11380147011458878No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT4760.11356289260910746No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT4730.1128471600926635No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC4690.11189285007073824No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4620.110222807532369No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC4580.10926849751044374No Hit
CCCTACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG4530.10807560998303714No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4510.10759845497207449No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAAATC4490.10712129996111187No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCAAAGCAGGCTTTCACTTT4480.10688272245563055No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC4430.10568983492822395No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAACGAAAAAAGGA4430.10568983492822395No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4420.10545125742274263No Hit
ACCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4410.10521267991726133No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4410.10521267991726133No Hit
GAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCA4390.10473552490629869No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4380.10449694740081736No Hit
GTTTTACTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAC4360.10401979238985473No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4310.10282690486244815No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCG4290.1023497498514855No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG4280.10211117234600417No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4250.10139543982956023No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC4240.1011568623240789No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)