FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533942
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11860.2221215038337497No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACAGT9650.18073124047181155No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAGACACCCGTG9280.17380164886822913No Hit
GTATGTAAATAGTGTCTCGTCTGGAAATAATGATTAACTACATGCTCTGG8820.1651864809286402No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAGTGTCTCGT8510.15938060688239547No Hit
GTCTTATATAAGAGAACTCTTGGACTTTCAGCAATGTCAACGACCGACCT8070.15114001146191908No Hit
TTCTTCTACTGTACCTAACTTTAATCCTAATTGGCAAACTCCTTCTTTTC8010.1500162939045814No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC7990.14964172138546883No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA7830.14664514123256833No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7410.13877911833120452No Hit
CTCTCAGAGGTGGAGATAAGGGAGTTGGCTGCCTTCCTGCCTGCCGATTG7270.13615711069741657No Hit
CTCCAAAAGACACCAAATATTCAAGAACAATGTCTCTTCCAAAAGTAAGA7140.13372238932318492No Hit
TCCAAAGAGTATGTAAATAGTGTCTCGTCTGGAAATAATGATTAACTACA6990.1309130954298407No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT6840.12810380153649648No Hit
ATGTTAATATGGGCCTAAAATTCAGACAACTCTTGTGGTTTCACATTTCT6740.12623093894093368No Hit
ATCTAAGGGCAAGTATTTGGTAACAGTGGGATAAAATCTAGCAGGCATAA6530.12229792749025176No Hit
CTATTATCCAGAGCATGTAGTTAATCATTATTTCCAGACGAGACACTATT6510.1219233549711392No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAGTGTCTCGTCTGGA6240.1168666259631196No Hit
ATGTAAATAGTGTCTCGTCTGGAAATAATGATTAACTACATGCTCTGGAT6010.11255904199332513No Hit
GTACACCATCTTGAGTCCATTTATGCCGCTGTTACCAATTTTCTTTTGTC6010.11255904199332513No Hit
TGTAAATAGTGTCTCGTCTGGAAATAATGATTAACTACATGCTCTGGATA5950.11143532443598743No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5800.1086260305426432No Hit
GCTTTATTCTTCTACTGTACCTAACTTTAATCCTAATTGGCAAACTCCTT5750.10768959924486181No Hit
CAATATGTTCCTGTGGCAAGGATCCCCAACTCCCAATTACATATCCCATG5650.10581673664929898No Hit
CCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTT5570.10431844657284874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)