FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546596
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20760.37980519432999876No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC16290.29802633023293257No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA16130.2950991225695029No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT16080.29418437017468113No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA12490.22850514822647805No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12280.2246631881682266No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA11390.20838059554039914No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA10620.19429340866014388No Hit
CACACAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC10460.19136620099671423No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10230.18715833998053408No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC10160.1858776866277836No Hit
CATTTAAACCCTCACAAAACGAAAAGATGGGGATATTCCCTTAACTTCAT10110.1849629342329618No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9550.174717707410958No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9500.17380295501613624No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8800.16099642148863147No Hit
CCTTCCAGGCGTCCGGCCAGTTGTCCTTGTGCGGGTTGAGGTCCCAATCT8640.1580692138252018No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTGACATT8560.15660560999348697No Hit
CCAACAAGGTGGGAGTGGGAGCATTCGGGCCAGGGTTCACCCCTCCCCAT8500.15550790711970083No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8490.15532495664073648No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCATATTTCCT8300.15184889754041375No Hit
CCCTTACAGTAAATGAAAATAGGAGACTAAAATTAATTATGCCTGCTAGG8290.1516659470614494No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8050.14727513556630492No Hit
GTATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8020.14672628412941185No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7990.1461774326925188No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC7850.14361612598701784No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTG7720.14123776976048122No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7710.14105481928151686No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7510.1373958097022298No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC7500.13721285922326545No Hit
ATGGCAGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCC7320.13391975060190708No Hit
TTTTACGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTT7300.13355384964397837No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7220.13209024581226353No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTCGTTT7120.13026074102262No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6980.12769943431711905No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT6970.1275164838381547No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6890.12605288000643985No Hit
GTACAATAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG6770.12385747425886762No Hit
GCACCGGACCATGCAAAGCCTGCACAACTCCTGCTCAAGGAACCTCTATG6710.12275977138508148No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTCTC6460.11818600941097264No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT6450.11800305893200828No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT6420.11745420749511523No Hit
CCTTGTTGGCGTCCGGCCAGTTGTCCTTGTGCGGGTTGAGGTCCCAATCT6220.11379519791582815No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6200.11342929695789944No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6090.11141684168929154No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6070.11105094073136283No Hit
CCTGGAAGGTGGGAGTGGGAGCATTCGGGCCAGGGTTCACCCCTCCCCAT6050.11068503977343412No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6010.10995323785757671No Hit
CTCAGCAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5930.10848963402586187No Hit
CCTCCTGCATACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5870.10739193115207575No Hit
GACAATGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAAC5850.10702603019414705No Hit
CCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGCCAAG5780.10574537684139658No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5760.10537947588346787No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5750.1051965254045035No Hit
CTATTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC5740.10501357492553914No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5720.10464767396761043No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC5710.10446472348864609No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5620.1028181691779669No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5570.10190341678314514No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5540.10135456534625206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)