FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507048
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10380.2047143465707389No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9870.19465612723055806No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9670.19071172748931067No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9370.18479512787743962No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8960.17670910840788248No Hit
ATCATCAGTTGGACCCTGCATTCAAAGCCAACTCAGAGAATCCAGATTGG8490.16743976901595115No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7960.15698710970164562No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7670.15126773007683691No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7110.14022341080134423No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7080.13963175084015714No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACCACA6410.12641801170697844No Hit
ACTGCTGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGATTTGTC6280.12385415187516763No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCGAGCTGTGCCTTGGGTGG5940.1171486723150471No Hit
GGCAACATACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAG5940.1171486723150471No Hit
TCCAAATTACTTCCCACCCAGGTGGCTAGATTCATTAACTCACCCCAACA5860.11557091241854815No Hit
CTCTTTGGAAGGCGGGGATCTTATACAAAAGAGAGTCCACACGTAGCGCC5690.11221817263848788No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT5640.11123207270317603No Hit
ACATTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCACTCCC5420.10689323298780393No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT5330.10511825310424258No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG5260.10373771319480603No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACGCTTCCGGA5210.10275161325949418No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5200.10255439327243179No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5160.10176551332418234No Hit
ATCCAGAGCATGTGGTTAATCATTACTTCCAGACGCGACATTATTTACAC5110.10077941338887048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)