FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507048
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10190.20096716681655388No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7870.1552121298180843No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7730.15245104999921114No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA7640.15067607011564982No Hit
ATCATCAGTTGGACCCTGCATTCAAAGCCAACTCAGAGAATCCAGATTGG6390.1260235717328537No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6330.12484025181047947No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5830.11497925245736104No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5520.1088654328584276No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5310.10472381313011785No Hit
CTCTTTGGAAGGCGGGGATCTTATACAAAAGAGAGTCCACACGTAGCGCC5280.10413215316893076No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT5110.10077941338887048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)