FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462745
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT15760.34057634334244563No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA14220.3072966752747193No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12750.2755297193918897No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12380.2675339549860074No Hit
ATGCAGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCGAGA11010.23792801651017298No Hit
GCGCCCTTACTCTCTTTTTTGCCGTCTGACTTCTTTCCTTCTATTCGCGA10060.21739835114371847No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG9380.20270343277615102No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9280.20054241536915582No Hit
GGATAATACGGTTTGATCCCTTTATCTAATGGCAAATATTTAGTAACCTT8400.1815254621875979No Hit
GAGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGA8160.1763390204108094No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8070.17439410474451372No Hit
AATCTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCC7970.17223308733751852No Hit
CCACTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT7580.16380511945023715No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA7450.1609957968211434No Hit
CCCAAAGTCAGGGCCCTGTACTTTCCTGCTGGTGGCTCCAGTTCAGGAAC7090.15321613415596064No Hit
CCACTAAGGGACACTCATCCTCAGGCCATGCAGTGGAATTCCACCACTTT6810.14716528541637403No Hit
GGACAAATTTGAGGACAACAGGTTGGTGAGTGACTGGAGATTTGGGACTG6730.14543647149077787No Hit
GGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCT6640.14349155582448217No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAATAATGATTAACTACA6440.13916952101049174No Hit
CGTTAATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCCT6440.13916952101049174No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG6020.13009324790111185No Hit
GTATTATCCAGAGCATGTAGTTAATCATTATTTCCAGACGCGACATTATT6010.12987714616041232No Hit
CCCTCATTGGTGGAGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTG5980.12922884093831377No Hit
CAGTAAGCCCTGCTCAGAATACTGTCTCAGCCATATCGTCAATCTTATCG5880.12706782353131854No Hit
GACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGGTGGAGTTCCACTGCA5850.12641951830922No Hit
GTTTCCTTCCCAAAAGTAAGACAGGAAATGTGAAACCACAATAATTGTCT5820.12577121308712141No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTCACTTCAT5800.12533900960572236No Hit
CTTTTATATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT5790.12512290786502284No Hit
CCTTATGTGTCCAAGGAATACTAACATTGAGATCCCCGAGATTGAGATCT5780.12490680612432332No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5540.11972036434753483No Hit
ATCCAGAGCATGTAGTTAATCATTATTTCCAGACGCGACATTATTTACAC5430.11734324519984007No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5430.11734324519984007No Hit
GTCCCGTGCTGGTCGTTGATGATCCTGGAATTAGGGGACAAACGGGCAAC5360.11583053301494343No Hit
GGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTG5300.11453392257074631No Hit
CTCTTTGGAAGGCGGGAATCTTATATAAAAGAGAGTCCACACGTAGCGCC5280.11410171908934727No Hit
CCCTTCTCTCCACCAATAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5220.11280510864515013No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5210.1125890069044506No Hit
CCCTTACAGTAAATGAAAAAAGAAGACTAAAATTAATTATGCCTGCTAGG5200.11237290516375109No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5170.11172459994165253No Hit
ATATTTCTGTCCCCAATCCCCTGGGATTCTTTCCCGATCATCAGTTGGAC5070.10956358253465731No Hit
GTCAACTATGTCAACGTTAATATGGGCCTAAAAATCAGACAATTATTGTG5050.10913137905325827No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5010.1082669720904602No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4980.10761866686836163No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAATGGCAAATATTTAGTA4830.1043771407578688No Hit
GTATCGGGATGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG4790.10351273379507071No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4780.1032966320543712No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGGGGACAAACGGGCAAC4760.10286442857297215No Hit
GCGCCCTTACTCTCTTTTTTGCCGTCTGACTTCTTTCCTTCTGTTCGCGA4760.10286442857297215No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4730.1022161233508736No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4640.10027120768457791No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)