FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414392
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC20720.500009652695998No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC19510.4708102473020715No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT19510.4708102473020715No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11560.27896291434197573No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10760.2596575223459912No Hit
TCCAAAGAGTGTGTAAATAATGTCGTGTCTGGAAGTAATGATTAACTACA10510.2536245873472461No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACGTTCCACCAAACTCTTCAAG10310.24879823934824996No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9740.23504314755111103No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8710.21018745535628103No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTTTCGCCAACTTACAAGGCC7850.1894341589605977No Hit
TATCCACATTGTGTGAAAGGTGCAGCAAACCCCAAAAGACCCACAATTCG7590.18315990656190273No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7580.18291858916195294No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCGTGT7510.1812293673623043No Hit
GGATAATACGGTTTGATCCCTTTATCTAATGGCAAATATTTAGTAACATT7490.18074673256240467No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACAACCGAG7290.17592038456340856No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7280.17567906716345877No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG6980.1684395451649646No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA6920.16699164076526574No Hit
CTCCAAAAGACACCAAATATTCAAGAACCGTTTCTCTCCCAAAAGTAAGA6870.16578505376551672No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6860.1655437363655669No Hit
CCCTTATCTCCACCTCTAAGGGACACGCATCCTCAGGCCATGCAGTGGAA6800.16409583196586808No Hit
GCCTGAGGATGCGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT6690.1614413405664202No Hit
CCTCTAAGGGACACGCATCCTCAGGCCATGCAGTGGAACTCCACCACGTT6280.15154732716847816No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC6200.14961678796887973No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTGACGT6200.14961678796887973No Hit
GCACGGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATA6110.14744493136933146No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC6100.14720361396938164No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6000.14479043996988358No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACACGACATTATTTACAC5990.1445491225699338No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5920.14285990077028515No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5800.13996409197088747No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5760.13899882237108824No Hit
GGAACATATTGTACAAAAAATCCAAATGTGTTTTAGGAAACTTCCTGTAA5760.13899882237108824No Hit
ATTCTATGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5700.1375509179713894No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5690.1373096005714396No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5620.13562037877179095No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA5620.13562037877179095No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5590.13489642657194154No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5580.13465510917199172No Hit
CGGTTGTTCCCCCTAGAAAACTGAGAGAAGTCCACCACGAGTCTAGACTC5520.1332072047722929No Hit
CCTTTCACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTAT5510.13296588737234308No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5380.1298287611729956No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5360.129346126373096No Hit
GTCCCGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5140.12403714357420027No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5110.12331319137435086No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACACGACATTATT5090.12283055657445124No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCTGAGACAGTA5080.12258923917450144No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGGCTCTCAGGCA5080.12258923917450144No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCGTGTCTGGA4990.12041738257495319No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAATGGCAAATATTTAGTA4940.11921079557520416No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT4850.1170389389756559No Hit
GGGTAAAGGTTCAGATACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGA4800.11583235197590687No Hit
CTGCTAGGCTGTACTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4790.11559103457595707No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4740.11438444757620803No Hit
TTGCTGCACCTTTCACACAATGTGGATATCCTGCTTTAATGCCTTTATAT4720.11390181277630843No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4670.11269522577655938No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4650.11221259097665978No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4630.11172995617676015No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGG4600.11100600397691074No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4560.11004073437711151No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4490.1083515125774629No Hit
CTGTTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCACAACTGTGCCAGC4470.10786887777756327No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG4410.10642097337786444No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG4410.10642097337786444No Hit
AGTTAAGGGAATATCCCCATCTTTTTGTTTTATGAGGGTTTAAATGTATA4390.10593833857796484No Hit
TCCTAGTACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCACT4370.10545570377806521No Hit
TCACCATACGGCTCTCAGGCAAGCTATTCTATGTTGGGGTGAGTTAATGA4360.1052143863781154No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4340.10473175157821579No Hit
CGTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAA4320.10424911677831619No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4280.10328384717851696No Hit
CCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGAAAC4280.10328384717851696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)