FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414392
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC15170.3660784957238557No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13160.31757369833394467No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12540.30261201953705674No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9920.23938686075020754No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACGTTCCACCAAACTCTTCAAG9350.22563176895306858No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8610.20777428135678294No Hit
TCCAAAGAGTGTGTAAATAATGTCGTGTCTGGAAGTAATGATTAACTACA8580.20705032915693355No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG7630.18412517616170196No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACAACCGAG7470.18026409776250507No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7040.16988744956466342No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6940.16747427556516536No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCGTGT6850.1653024189656171No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6590.15902816656692215No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC6570.15854553176702255No Hit
CTCCAAAAGACACCAAATATTCAAGAACCGTTTCTCTCCCAAAAGTAAGA6550.15806289696712292No Hit
GGATAATACGGTTTGATCCCTTTATCTAATGGCAAATATTTAGTAACATT6540.15782157956717313No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6070.14647966176953223No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6060.14623834436958244No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACACGACATTATTTACAC5930.14310121817023494No Hit
GTCCCGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5630.13586169617174076No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACACGACATTATT5500.1327245699723933No Hit
TATCCACATTGTGTGAAAGGTGCAGCAAACCCCAAAAGACCCACAATTCG5460.13175930037259406No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA5450.13151798297264425No Hit
CCTCTAAGGGACACGCATCCTCAGGCCATGCAGTGGAACTCCACCACGTT5390.13007007857294542No Hit
GCCTGAGGATGCGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT5350.1291048089731462No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5350.1291048089731462No Hit
CCCTTATCTCCACCTCTAAGGGACACGCATCCTCAGGCCATGCAGTGGAA5270.12717426977354773No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC5160.12451977837409989No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5140.12403714357420027No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGGCTCTCAGGCA5110.12331319137435086No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4880.11776289117550531No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTTTCGCCAACTTACAAGGCC4830.11655630417575628No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAATGGCAAATATTTAGTA4800.11583235197590687No Hit
ATTCTATGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA4770.11510839977605744No Hit
CCTTTCACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTAT4760.11486708237610765No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC4710.11366049537635861No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4710.11366049537635861No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4670.11269522577655938No Hit
GGAACATATTGTACAAAAAATCCAAATGTGTTTTAGGAAACTTCCTGTAA4610.11124732137686055No Hit
GCACGGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATA4500.10859282997741268No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCTGAGACAGTA4500.10859282997741268No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4430.10690360817776404No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4430.10690360817776404No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC4410.10642097337786444No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4280.10328384717851696No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4240.10231857757871773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)