FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476462
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17600.3693893741788432No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC13460.2824989191163199No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA9780.20526295906074357No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9300.19518870340132058No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9100.190991096876561No Hit
CTCTTACAGTGAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG8810.18490456741565958No Hit
GTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAATTCTTTGGCATACT8650.18154648219585193No Hit
AGTCTAGACTCCGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8410.17650935436614043No Hit
GGAACATATTGTACAAAAATTGAGACAATGTTTTCGGAAACTTCCTGTAA6860.14397790379925365No Hit
GTATACATGCATATAAAGGCATTAAGGCTGGATAACCACATTGTGTAAAA6850.1437680234730157No Hit
CTCTAGAACAACTTCTCTTCCAAACATAAGACAGGAAAGGTGAAACCACA6460.1355826907497345No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACCGTATGTTCAGG5930.12445903345912161No Hit
CTTTTACACAATGTGGTTATCCAGCCTTAATGCCTTTATATGCATGTATA5790.1215207088917899No Hit
CTCCAAAAGACACCAAATACTCTAGAACAACTTCTCTTCCAAACATAAGA5740.1204713072606No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5720.12005154660812406No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5470.11480453845217457No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA5460.11459465812593658No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC5420.11375513682098468No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC5390.11312549584227075No Hit
GCCTAGCAGCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5370.11270573518979478No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA5240.10997729094870107No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG5200.10913776964374913No Hit
CCCAAAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCAAGTTCCGGAAC5160.10829824833879721No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5140.10787848768632126No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT5070.10640932540265541No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5060.10619944507641742No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTACCGC5050.10598956475017945No Hit
ACTACACACAGCGCCTCATTCTGTGGGTCACCATATTCTTGGGAACAAGA4910.10305124018284773No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTAAGGCA4830.10137219757294391No Hit
GCTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4810.10095243692046796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)