FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527029
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT21540.4087061622794951No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG16930.32123469486498846No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA16520.31345523680860066No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT15840.30055272100776237No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA14120.26791694574681846No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13710.2601374876904307No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13100.24856317204556105No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA12900.2447683144570792No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG12830.24344011430111057No Hit
ATATAAAAGAGAGTCCACACGTAGTGCCTCATTTTGCGGGTCACCATATT11640.22086071164964358No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG11230.21308125359325575No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT11180.2121325391961353No Hit
GTATATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG11020.20909665312534984No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAAAGTCCAA11000.20871716736650167No Hit
GTTTACAGGAAGCTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT10950.2077684529693812No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10760.20416333826032343No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA10680.2026453952249307No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA10580.20074796643068976No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT9860.18708647911215512No Hit
AGTCTAGACTCTGTGGTATTGTGAGGACTTTTGTCAACAAGAAAAACCCC9480.17987624969403962No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG9470.1796865068146155No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT9090.1724762773965No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8980.17038910572283497No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8750.16602501949608087No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGTGCC8590.16298913342529536No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT8430.15995324735450991No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAGCTTCCTGTAA8230.15615838976602806No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGACTTTATTCTTCTACGG8210.15577890400717986No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT7950.15084558914215346No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC7940.15065584626272938No Hit
GTTCATATACTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA7930.1504661033833053No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7890.1497071318656089No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG7830.14856867458906436No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7780.1476199601919439No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC7720.14648150291539935No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7460.14154818805037292No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7230.13718410182361881No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT7220.13699435894419473No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7050.13376872999398515No Hit
ATGTTAATATGGGCCTAAAATTCAGGCAACTATTGTGGTTTCACGTTTCC6890.13073284392319967No Hit
ATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACAGGAAACGTGAAACCAC6850.12997387240550332No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC6810.12921490088780693No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6740.12788670073183828No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG6630.12579952905817327No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT6450.1223841572285396No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6430.12200467146969143No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6400.12143544283141913No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC6380.12105595707257097No Hit
CAATAGGCCTGTTTACAGGAAGCTTCCTAAAACACATTTTGATTTTTTGT6320.11991749979602641No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA6290.11934827115775413No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6280.11915852827833003No Hit
ACTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6200.11764058524293729No Hit
TATTAACATTGACATAGCCGACTACTAATTCCCTTGATGCAGGATCTTCC6190.1174508423635132No Hit
GTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT6170.11707135660466501No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6160.11688161372524093No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA6120.11612264220754456No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAAC6070.1151739278104241No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG6050.1147944420515759No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT6040.11460469917215182No Hit
AGTCTAGACTCTGTGGTATTGTGAGGACTTTTGTCAACGAGAAAAACCCC6030.11441495629272772No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6000.11384572765445544No Hit
CACATAAGGTGGGAAACTTTACGGGACTTTATTCTTCTACGGTACCTTGC5980.11346624189560725No Hit
CAATCACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5940.1127072703779109No Hit
CTCTTATACAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTTTC5930.1125175274984868No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA5890.11175855598079044No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5860.11118932734251814No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA5840.11080984158366998No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT5810.11024061294539768No Hit
CTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC5800.1100508700659736No Hit
ATATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG5770.10948164142770132No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5640.1070149839951881No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC5630.10682524111576402No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT5630.10682524111576402No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG5620.10663549823633994No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5570.10568678383921948No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5510.10454832656267492No Hit
CTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT5510.10454832656267492No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGACTTTATTC5490.10416884080382673No Hit
GTATAAAGAATTTGGAGCTTCTACGGAGTTACTCTCTTTTTTGCCTTCTG5490.10416884080382673No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5470.10378935504497856No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5460.10359961216555445No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5430.10303038352728218No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC5420.10284064064785808No Hit
CATAAGGTGGGAAACTTTACGGGACTTTATTCTTCTACGGTACCTTGCTT5400.1024611548890099No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGC5380.10208166913016171No Hit
ATCCTGCATCAAGGGAATTAGTAGTCGGCTATGTCAATGTTAATATGGGC5330.10113295473304126No Hit
TATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5320.10094321185361715No Hit
GCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG5320.10094321185361715No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5290.10037398321534488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)