FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592849
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27030.4559339730690277No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC22210.37463165156726247No Hit
GTTAATATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC20420.34443846578133724No Hit
CCTTTGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGT19920.3360046150031458No Hit
CTTTCTCTAAGGGCAAATATTTAGTAACCTTGGGATAAAACCTAGCAGGC11100.18723148727584932No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT11100.18723148727584932No Hit
TCTCAGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10430.17593012723307286No Hit
CTTTTATATAAGACACCCGCCTTCCAAAGAATGTGTAAATAATGTCTCGT9200.15518285431872197No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9040.15248402206970071No Hit
CATCTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8380.14135133904248806No Hit
CCCTTACAGTAAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG8350.14084530799579656No Hit
GGAATATGATAAAACGCCGCAGATACATCCAGCGATAACCAGGACAAATT8350.14084530799579656No Hit
CCTTCAAATGAATGTCAGGAAAAGAAGGAGTTTGCCATTTAGGATTAAAG7510.126676438688435No Hit
ATCTATCTGTCCCCAATCCCTTGGGATTCTTCCCCGATCATCAGTTGGAC7460.12583305361061586No Hit
GATCTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA7450.12566437659505203No Hit
GTCTATATGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT7380.12448363748610523No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC7310.12330289837715844No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7230.12195348225264781No Hit
ATCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT7210.12161612822152014No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTA7150.12060406612813719No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7130.12026671209700952No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7120.1200980350814457No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGT7020.11841126492580742No Hit
TAGAGAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC7010.11824258791024359No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC6950.11723052581686061No Hit
GGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTA6800.1147003705834032No Hit
CTCTTGGACTGTTAGCAATGTCAACAACCGACCTTGAGGCATACTTCAAA6360.10727858189859475No Hit
GGTCTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGAC6350.10710990488303092No Hit
CATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGAGGG6300.10626651980521179No Hit
ACTATATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA6260.10559181174295647No Hit
CGCTTAGAGTCCTCTTATGCAACAGTCCAAGAGTCCTCTTATGCAAGACC6220.10491710368070116No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6150.10373636457175436No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6080.10255562546280755No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT6060.1022182714316799No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)