FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490767
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25770.525096430689105No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13350.2720231800426679No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11450.23330827052348674No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT11400.2322894571150872No Hit
CATGTATACAAGCCAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11210.22841796616316906No Hit
GGATAATAGGGTTTGATCCCTTTATCTAAGGGCAAGTATTTAGTAACATT10420.21232071431045688No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA10040.20457773240662064No Hit
GACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTAACAGGA9420.1919444461424668No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACGCTTCCGGA9250.18848048055390848No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9080.18501651496535015No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8720.17768105842487372No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7860.16015746780040221No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG7830.15954617975536253No Hit
ACTAAATACTTGCCCTTAGATAAAGGGATCAAACCCTATTATCCAGAGCA7400.15078438444312678No Hit
CCTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTGCT7260.14793170689960816No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6850.13957743695073221No Hit
CCCTTACAGTAAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGA6700.1365209967255337No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCGAGGAACCTCTATG6620.1348908952720945No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACACATTCTGATTTTTTGT6470.13183445504689598No Hit
TCACCATACTGCACTCAGGCAAGCCATTGTGTGTTGGGGTGAGTTAATGA6350.12938930286673717No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6230.12694415068657836No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGGCGCGACATTACT6170.12572157459649896No Hit
GTAGTCAACTATGTCAACGTTAATATGGGCATAAAAATCAGACAGCTATT6130.12490652386977935No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6080.12388771046137984No Hit
ATATGTTCTTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA6070.12368394777969995No Hit
ATACTGCACTCAGGCAAGCCATTGTGTGTTGGGGTGAGTTAATGAATCTA5960.12144255828122102No Hit
ACGTTAATATGGGCATAAAAATCAGACAGCTATTGTGGTTTCACATTTCC5920.12062750755450141No Hit
TCTATCAACAATGTCCTGCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5910.12042374487282154No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG5780.1177748300109828No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG5680.11573720319418379No Hit
TCCAAAGGGTGTGTAAGTAATGTCGCGCCTGGAAGTAATGATTAACTACA5650.11512591514914408No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACAGAAT5580.11369957637738477No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5460.11125442419722598No Hit
GCTTTAGGGCATGGACATTGACACCTATAAAGAATTTGGAGCTTCTGTGG5450.11105066151554607No Hit
CGTTAATATGGGCATAAAAATCAGACAGCTATTGTGGTTTCACATTTCCT5410.11023561078882646No Hit
CCAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5400.11003184810714657No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5320.10840174665370736No Hit
GTATTTAGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC5290.10779045860866765No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5250.10697540788194805No Hit
CATCTATCAACAATGTCCTGCTGCAAATGAATGTCAGGAAAAGAAGGAGT5170.10534530642850885No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCGCAAGCCATGCAGTGGAA5160.10514154374682895No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5120.10432649302010934No Hit
GTACACAAAATCAGAATGTGTTTTAGGAAACTTCCTGTTAACAGGCCTAT4980.10147381547659072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)