FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490767
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25470.518983550238708No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12600.25674097891667536No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11970.24390392997084157No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA10960.22332389912117154No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10740.21884112012421375No Hit
GGATAATAGGGTTTGATCCCTTTATCTAAGGGCAAGTATTTAGTAACATT10120.20620783386005986No Hit
CATGTATACAAGCCAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9360.1907218700523874No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8200.16708539897751887No Hit
GACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTAACAGGA8050.16402895875232035No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7750.15791607830192333No Hit
CCTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTGCT7680.156489739530164No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACGCTTCCGGA7270.14813546958128807No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG7240.14752418153624836No Hit
CCCTTACAGTAAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGA6970.14202258913089102No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6930.14120753840417144No Hit
GTATTTAGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC6850.13957743695073221No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6680.13611347136217392No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6480.13203821772857588No Hit
ACTAAATACTTGCCCTTAGATAAAGGGATCAAACCCTATTATCCAGAGCA6170.12572157459649896No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6120.12470276118809943No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6060.12348018509802004No Hit
GTAGTCAACTATGTCAACGTTAATATGGGCATAAAAATCAGACAGCTATT5800.11818235537434262No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCGAGGAACCTCTATG5650.11512591514914408No Hit
ATATGTTCTTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA5650.11512591514914408No Hit
GTACACAAAATCAGAATGTGTTTTAGGAAACTTCCTGTTAACAGGCCTAT5560.11329205101402498No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5550.11308828833234508No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGGCGCGACATTACT5520.11247700028730538No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5520.11247700028730538No Hit
AATCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5430.11064313615218628No Hit
ACGTTAATATGGGCATAAAAATCAGACAGCTATTGTGGTTTCACATTTCC5410.11023561078882646No Hit
GCTTTAGGGCATGGACATTGACACCTATAAAGAATTTGGAGCTTCTGTGG5370.10942056006210688No Hit
ATCTAAGGGCAAGTATTTAGTAACATTGGGATAAAATCTAGCAGGCATAA5340.10880927201706717No Hit
ATACTGCACTCAGGCAAGCCATTGTGTGTTGGGGTGAGTTAATGAATCTA5310.10819798397202746No Hit
TCCAAAGGGTGTGTAAGTAATGTCGCGCCTGGAAGTAATGATTAACTACA5270.10738293324530786No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG5140.10473401838346916No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAATTAGGGGAGGAGGTTA5110.10412273033842943No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACACATTCTGATTTTTTGT5060.10310391693002993No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT5040.10269639156667014No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)