FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202324
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17680.8738459105197605No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG8060.3983709297957731No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC4320.21351891026274686No Hit
GGATAATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTACTGTT4190.20709357268539572No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT3720.1838635060595876No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT3620.17892093869239437No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCAAGTAT3470.17150708764160455No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG3310.1635989798540954No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3140.15519661532986695No Hit
GGACATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACGGTTAACG3100.15321958838298966No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT3100.15321958838298966No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC3050.15074830469939304No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT2760.13641485933453273No Hit
CTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT2720.13443783238765544No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTAGCTCTTCCAAAAGTAAGA2710.13394357565093612No Hit
CTCTTACGGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG2700.1334493189142168No Hit
GCTTACTACTGCTTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC2670.13196654870405886No Hit
ATGTAGGCCCTCTTACGGTTAACGAAAAAAGGAGATTAAAATTGATTATG2660.1314722919673395No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA2660.1314722919673395No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2600.1285067515470236No Hit
ACAATATGTAGGCCCTCTTACGGTTAACGAAAAAAGGAGATTAAAATTGA2590.12801249481030424No Hit
ATATAAGACCTTGGGCAAGACTTGGCGGGCGTTCACGGTGGTTTCCATGC2540.12554121112670766No Hit
GTTCTATCCTAACAGTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC2520.12455269765326901No Hit
CTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT2520.12455269765326901No Hit
CTCAAGAACAGTAGCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA2490.12306992744311104No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC2430.12010438702279513No Hit
ATCCTAACAGTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCATAT2410.11911587354935649No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG2390.11812736007591783No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC2390.11812736007591783No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACGGTTAACGAAAAAAGGA2380.11763310333919852No Hit
ATATGGTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA2370.11713884660247918No Hit
ATATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT2350.11615033312904054No Hit
GTTCAGGATAATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA2320.11466756291888258No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC2310.11417330618216327No Hit
CCTACCGCTTACAGACCAGCAAATGCCCCTATCTTATCAACACTTCCGGA2210.10923073881497006No Hit
ATTTGGTACTGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTC2210.10923073881497006No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG2160.10675945513137343No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA2160.10675945513137343No Hit
CTTACTAACCTCTTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTC2110.10428817144777683No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA2040.1008283742907416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)