FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501872
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAGTAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG11580.230736123951924No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT10660.21240475659132207No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT9560.19048681735581982No Hit
GTGCTGGATATTAGACCTTTGTTGGATGCATAGTTTGCAAATATTTTCTC9310.18550546752956928No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9120.18171964166161889No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9090.18112187968246884No Hit
GTGCTGGGAGGATTTTCCATTCTTCAAGTGAAACTGAAATTGAGACTTTT8830.1759412758631683No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8450.16836962412726753No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8410.16757260815506742No Hit
GTGCTGGCATAAAAACAGGCATATAGACCAATGGAAAAAATAAAGAACTC7200.14346287499601493No Hit
GTGTAAGGAAGGAGTCCAGATTCTCTCTTCTGCATATGGCTAGCCAGTTA7200.14346287499601493No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6800.1354927152740141No Hit
CTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC6560.1307106194408136No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5960.11875537985781236No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5930.11815761787866227No Hit
GCCTAGCAGCCATGGAAAAGCGGTGTATTTCCGAGAAAGGACAACAGAGT5820.11596582395511205No Hit
GGTTAGGATAAAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG5560.11078522013581152No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG5550.1105859661427615No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG5460.10879268020531131No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5340.10640163228871106No Hit
ATATAGACCAATGGAAAAAATAAAGAACTCAGAAGTAAATCTATGCACTT5190.10341282239296075No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5150.10261580642076068No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG5040.10042401249721045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)