FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524305
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA28350.5407158047319784No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTAAAAGTCCAA22950.4377223181163636No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT20290.3869884895242273No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19230.3667712495589399No Hit
TCTCATGTTCATGTCCTACTATTCAAGCCTCCAAGCTGTGCCTTGGGTGG19140.3650546914486797No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATAACCACATTGTGTGAAA16600.31660960700355706No Hit
GTCTTTGCAGTATGCCTCAAGGTCGGTCGTTGACATTGCTAAAAGTCCAA16220.3093619172046805No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATAATTAACCC15750.3003976692955436No Hit
CTAAACAACCAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGG15510.29582018100151625No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCT13570.25881881729146206No Hit
GTGGAATTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGAGA13450.25653007314444837No Hit
CATCTAAACCCTCATAAAACAAAAAGATGGGGCTATTCCCTTAACTTCAT12950.2469936391985581No Hit
TCTCTTGTTCATGTCCTACTATTCAAGCCTCCAAGCTGTGCCTTGGGTGG12860.24527708108829782No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACACTGTTCACCTCACCATACGG12790.2439419803358732No Hit
GGTTTTTTAGAAAAGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTA12640.2410810501521061No Hit
GTATAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT12410.2366942905369966No Hit
GTTTTATACAGCAACAGGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTC12410.2366942905369966No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC12130.23135388752729805No Hit
TTTCCGGGACAGGACGACAGAATTGTCGGTCCCGAGAAGTTTCGCTCCAG12110.23097243016946242No Hit
CTTTGCAGTATGCCTCAAGGTCGGTCGTTGACATTGCTAAAAGTCCAAGA11950.22792077130677754No Hit
GTGTGAAAGGAGCAGCAAATCCCAAAAGACCTACAATTCGTTGACACACT11440.21819360868196944No Hit
GTCCCGTAAATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACT10500.20026511286369578No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC10340.19721345400101087No Hit
GTATGGATCGGCAGTGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC9970.19015649288105205No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC9950.18977503552321645No Hit
CCCGTAAATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGG9370.17871277214598372No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG9320.1777591287513947No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG9240.17623329932005227No Hit
TAACCACATTGTGTGAAAGGAGCAGCAAATCCCAAAAGACCTACAATTCG9140.1743260125308742No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG9130.1741352838519564No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG9090.17337236913628518No Hit
TTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC9060.17280018309953177No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTAGGTCT8870.16917633820009345No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT8830.16841342348442223No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACGG8740.16669686537416198No Hit
CCTTTCACACAATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTAT8440.1609750050066278No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC8160.15563460199692927No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC8120.15487168728125805No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC8060.1537273152077512No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG7990.15239221445532658No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA7980.15220148577640877No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACATAAACC7970.15201075709749096No Hit
CATATAAAGGCATTAAAGCAGGATAACCACATTGTGTGAAAGGAGCAGCA7900.15067565634506633No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT7850.1497220129504773No Hit
ATTTCCGGGACAGGACGACAGAATTGTCGGTCCCGAGAAGTTTCGCTCCA7750.14781472616129926No Hit
GTTTAGATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA7750.14781472616129926No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA7720.14724254012454585No Hit
GTTTACAGGAAGTTTTCTAAAACATTGTTTGATTTTCAACACAATATGTT7500.1430465091883541No Hit
CCTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTTTTGCCTTCT7360.14037630768350481No Hit
GGATAACCACATTGTGTGAAAGGAGCAGCAAATCCCAAAAGACCTACAAT7340.13999485032566922No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACATTGTTTGATTTTCAAC7280.1388504782521624No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG7270.1386597495732446No Hit
CCTACCCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA7150.13637100542623093No Hit
CTTTAGGGCATGGACATTGACACCTATAAAGAATTTGGAGCTTCTGTGGA7090.13522663335272408No Hit
TTTCCGGGACAGGACGACAGAATTGTCGGTCCCGATAAGTTTCGCTCCAG7060.13465444731597065No Hit
CTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTAAAAGTCCAAGA7060.13465444731597065No Hit
GGTTTAGATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6880.13122133109545017No Hit
GGTTCCGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAG6880.13122133109545017No Hit
GGTTTTATACAGCAACAGGAGGGAAACATAGAGGTTCCTTGAGCAGGAGT6870.13103060241653236No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6870.13103060241653236No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6830.13026768770086114No Hit
GTACGGCAGACGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG6810.12988623034302554No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTAGGTCTTTTGGGATTT6650.12683457148034066No Hit
GTCGTTGACATTGCTAAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG6620.12626238544358723No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA6590.1256901994068338No Hit
CCGCTTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACACTGTTCA6560.12511801337008038No Hit
CCTCAAGGTCGGTCGTTGACATTGCTAAAAGTCCAAGAGTCCTCTTATGC6510.12416436997549136No Hit
GCCTAATCATCTCATGTTCATGTCCTACTATTCAAGCCTCCAAGCTGTGC6390.12187562582847769No Hit
CCCGATAAGTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCAGCTAGGA6370.1214941684706421No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTC6230.11882396696579281No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT6220.118633238286875No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA6180.11787032357120379No Hit
GCCTTAGAGTCTCCGGAACACTGTTCACCTCACCATACGGCACTCAGGCA6170.11767959489228598No Hit
ATGCAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC6140.11710740885553256No Hit
TCCCGATACAGAGCAGAAGCGGTGTCGAGAAGATCTCGAATAGAAGGAAA6090.11615376546094353No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6020.11481866470851888No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG6020.11481866470851888No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5860.111767005845834No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG5840.1113855484879984No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAAGCGGTGTCGAGAAGATCTCGAA5810.11081336245124498No Hit
GTAGAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCAGGATTCAGCGCCG5780.11024117641449158No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5740.10947826169882034No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACTTTACCCCAG5700.10871534698314912No Hit
CTACTATTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGAC5700.10871534698314912No Hit
ATATGTTCCTGGGGTAAAGTGCCCCAACTTCCAATTACATATCCCATGAA5670.1081431609463957No Hit
GTTTACGTCCCCTCGGCGCTGAATCCTGCGGACGACCCCTCCCGGGGCCG5660.10795243226747789No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTGTGCCAAGTG5600.10680806019397107No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC5590.10661733151505326No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC5570.10623587415721765No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG5540.10566368812046424No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5530.10547295944154643No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5500.10490077340479301No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG5490.1047100447258752No Hit
TTGGTAGTTGGGGCACTTTACCCCAGGAACATATTGTGTTGAAAATCAAA5490.1047100447258752No Hit
GTTTTGTACAGCAACAGGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTC5460.10413785868912179No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5460.10413785868912179No Hit
CCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAAC5450.10394713001020399No Hit
GGAACATATTGTGTTGAAAATCAAACAATGTTTTAGAAAACTTCCTGTAA5420.10337494397345055No Hit
ACCACATTGTGTGAAAGGAGCAGCAAATCCCAAAAGACCTACAATTCGTT5360.10223057189994372No Hit
GCTTTGGGGCATGGACATTGACACCTATAAAGAATTTGGAGCTTCTGTGG5340.10184911454210813No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTACGGGACAGGACGACAGAAT5340.10184911454210813No Hit
GATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAAGGGAC5320.1014676571842725No Hit
GGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGA5280.1007047424686013No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCGGTGGGCGTTC5270.10051401378968348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)