FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527057
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG36690.6961296406271048No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT24870.4718654718559852No Hit
GGATATTATTAATAGATGTCAACAATATGTAGGCCCGCTTACAGTTAATG22440.42576040162638956No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT19000.3604923186676204No Hit
ACAATATGTAGGCCCGCTTACAGTTAATGAAAAAAGGAGATTACAATTGA16760.31799217162470095No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG15010.2847889317474201No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14710.27909694776845767No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCGCTTACAGTTAATGAAAAA14660.27814828377196393No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA14230.2699897734021178No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT13820.26221072863086914No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT13590.257846874246998No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATTAGACAACTATT13550.2570879430498029No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT13370.2536727526624255No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT13140.24930889827855432No Hit
GTGTAAAGGGAGCGGCAAAGCCCAAAAGCCCCACAATTCGTTGACATACT12910.24494504389468313No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC12070.22900748875358834No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA11570.21952084878865097No Hit
ATGTAGGCCCGCTTACAGTTAATGAAAAAAGGAGATTACAATTGATTATG11550.21914138319005344No Hit
TATTAATAGATGTCAACAATATGTAGGCCCGCTTACAGTTAATGAAAAAA11530.21876191759145594No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTGTAATCTCCTTTTTTCA10750.20396275924615365No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA10250.19447611928121628No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG10100.19163012729173504No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10000.18973279929874756No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA9920.1882149369043576No Hit
CTATTAATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG9910.18802520410505885No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9740.18479974651698014No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9390.17815909854152395No Hit
GATTACAATTGATTATGCCTGCTAGGTTCTATCCTAACCGTACCAAATAT9360.17758990014362774No Hit
ATGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCC9170.17398497695695153No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC9050.17170818336536656No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8860.16810326017869034No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8840.16772379458009284No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8710.16525726818920913No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8640.1639291385941179No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC8630.16373940579481916No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC8570.16260100899902669No Hit
CAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG8540.16203181060113042No Hit
ATATGTAGGCCCGCTTACAGTTAATGAAAAAAGGAGATTACAATTGATTA8510.16146261220323418No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC8430.15994474980884418No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT8420.15975501700954545No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA8400.15937555141094797No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC8380.15899608581235047No Hit
GGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT8350.15842688741445424No Hit
GGATAGAACCTAGCAGGCATAATCAATTGTAATCTCCTTTTTTCATTAAC8350.15842688741445424No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA8240.156339826622168No Hit
ATTATTAATAGATGTCAACAATATGTAGGCCCGCTTACAGTTAATGAAAA8160.15482196422777803No Hit
CAATAGGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT8070.1531143690340893No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7960.15102730824180308No Hit
CGCTTACAGTTAATGAAAAAAGGAGATTACAATTGATTATGCCTGCTAGG7910.15007864424530934No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7820.1483710490516206No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7730.1466634538579319No Hit
ATTAATAGATGTCAACAATATGTAGGCCCGCTTACAGTTAATGAAAAAAG7650.1451455914635419No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA7390.14021253868177447No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7370.13983307308317697No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA7170.136038417097202No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATTAGACA7150.13565895149860452No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG7090.13452055470281202No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7080.13433082190351328No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC7040.13357189070631828No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG6980.1324334939105258No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6970.13224376111122707No Hit
GTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCCTG6950.13186429551262957No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6810.1292080363224471No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG6780.12863883792455086No Hit
CTCATAAGGTGGGAAACTTCACTGGGCTTTATTCTTCTACTGTACCTGTC6720.12750044112875838No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTCCGAAAAC6600.1252236475371734No Hit
TAATAGATGTCAACAATATGTAGGCCCGCTTACAGTTAATGAAAAAAGGA6350.12048032755470471No Hit
TTACAATTGATTATGCCTGCTAGGTTCTATCCTAACCGTACCAAATATTT6320.11991112915680846No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACCACT6290.11934193075891222No Hit
GATGTCAACAATATGTAGGCCCGCTTACAGTTAATGAAAAAAGGAGATTA6210.11782406836452224No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6180.11725486996662601No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT6160.1168754043680285No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG6040.11459861077644352No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT6010.1140294123785473No Hit
CCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC5990.1136499467799498No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA5960.11308074838205355No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATAA5930.11251154998415731No Hit
ATGTCAATGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCAC5910.11213208438555981No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC5850.11099368758976734No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA5840.11080395479046859No Hit
GTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATA5800.1100450235932736No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT5760.10928609239607862No Hit
CTCTAAGGCCTCCCGATAGAGAGCAGAGGCGGTGTCGAGGAGATCACGAA5720.10852716119888361No Hit
CCCTTGGACTCATAAGGTGGGAAACTTCACTGGGCTTTATTCTTCTACTG5720.10852716119888361No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTCACTGGGCTTTATTC5700.10814769560028611No Hit
GATTATGCCTGCTAGGTTCTATCCTAACCGTACCAAATATTTGCCCTTAG5700.10814769560028611No Hit
GGCCTACATATTGTTGACATCTATTAATAATATCCTCTTTCAAATGAATG5690.10795796280098736No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5650.10719903160379238No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5650.10719903160379238No Hit
GTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAATAGTTGTCT5590.1060606348079999No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5590.1060606348079999No Hit
ACATATTGTTGACATCTATTAATAATATCCTCTTTCAAATGAATGTGAGG5570.1056811692094024No Hit
CAACAATATGTAGGCCCGCTTACAGTTAATGAAAAAAGGAGATTACAATT5470.10378384121641493No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTGTAA5430.10302491001921994No Hit
GCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAGCACTAAGGCA5410.10264544442062243No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5370.10188651322342744No Hit
ACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATA5310.10074811642763497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)