FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527057
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG45070.8551257264394553No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT22730.43126265280605325No Hit
GGATATTATTAATAGATGTCAACAATATGTAGGCCCGCTTACAGTTAATG22350.4240528064327008No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT19290.36599456984728407No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA15910.3018648836843074No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCGCTTACAGTTAATGAAAAA15330.29086038132498004No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT14630.27757908537406767No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG14560.27625095577897646No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATTAGACAACTATT14270.2707487045993128No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT13910.2639183238245579No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13730.26050313343718046No Hit
ACAATATGTAGGCCCGCTTACAGTTAATGAAAAAAGGAGATTACAATTGA13660.2591750038420892No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT13240.25120622627154177No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT13080.24817050148276182No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC13020.24703210468696937No Hit
GTGTAAAGGGAGCGGCAAAGCCCAAAAGCCCCACAATTCGTTGACATACT12860.2439963798981894No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA11740.22274630637672965No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA11290.214208330408286No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTGTAATCTCCTTTTTTCA11100.21060340722160983No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG10970.2081368808307261No Hit
TATTAATAGATGTCAACAATATGTAGGCCCGCTTACAGTTAATGAAAAAA10830.20548062164054362No Hit
CTATTAATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG10410.19751184406999622No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA10290.19523505047841125No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC10160.19276852408752754No Hit
ATGTAGGCCCGCTTACAGTTAATGAAAAAAGGAGATTACAATTGATTATG10030.19030199769664383No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9960.1889738681015526No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA9880.1874560057071626No Hit
ATGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCC9680.18366134972118764No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9650.18309215132329143No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9610.18233322012609643No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC9570.18157428892890143No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9370.17777963294292648No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC9200.17455417535484777No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9060.1718979161646653No Hit
ATATGTAGGCCCGCTTACAGTTAATGAAAAAAGGAGATTACAATTGATTA8880.16848272577728785No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8770.16639566498500163No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT8760.16620593218570287No Hit
GGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT8740.16582646658710537No Hit
GATTACAATTGATTATGCCTGCTAGGTTCTATCCTAACCGTACCAAATAT8630.16373940579481916No Hit
GGATAGAACCTAGCAGGCATAATCAATTGTAATCTCCTTTTTTCATTAAC8470.16070368100603918No Hit
CAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG8300.15747822341796047No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC8210.15577062822427176No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC8080.15330410183338805No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8060.15292463623479055No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC7970.1512170410411018No Hit
CAATAGGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT7970.1512170410411018No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATTAGACA7930.1504581098439068No Hit
ATTATTAATAGATGTCAACAATATGTAGGCCCGCTTACAGTTAATGAAAA7930.1504581098439068No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA7810.14818131625232187No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7740.14685318665723063No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7710.14628398825933436No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7430.14097146987896947No Hit
CGCTTACAGTTAATGAAAAAAGGAGATTACAATTGATTATGCCTGCTAGG7330.13907414188598197No Hit
GTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCCTG7130.13527948590000702No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7040.13357189070631828No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG6990.13262322670982454No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6990.13262322670982454No Hit
ATGTCAATGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCAC6950.13186429551262957No Hit
ATTAATAGATGTCAACAATATGTAGGCCCGCTTACAGTTAATGAAAAAAG6880.13053616591753833No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6860.13015670031894083No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG6770.12844910512525212No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT6690.12693124273086212No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6650.12617231153366715No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT6550.12427498354067967No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA6510.12351605234348469No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC6500.12332631954418592No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6500.12332631954418592No Hit
CTCATAAGGTGGGAAACTTCACTGGGCTTTATTCTTCTACTGTACCTGTC6480.12294685394558844No Hit
GTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAATAGTTGTCT6450.12237765554769219No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6290.11934193075891222No Hit
GTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATA6220.118013801163821No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATAA6220.118013801163821No Hit
GTCTTTGGGTATACATTTAAACCCTACTAAAACTAAACGTTGGGGCTACT6090.11554727477293729No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG6000.11383967957924854No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA5920.11232181718485855No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC5910.11213208438555981No Hit
TAATAGATGTCAACAATATGTAGGCCCGCTTACAGTTAATGAAAAAAGGA5860.11118342038906608No Hit
GGCCTACATATTGTTGACATCTATTAATAATATCCTCTTTCAAATGAATG5810.11023475639257234No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTGTAA5790.10985529079397484No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5740.1089066267974811No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACCACT5700.10814769560028611No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTCCGAAAAC5700.10814769560028611No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT5680.10776823000168863No Hit
TTACAATTGATTATGCCTGCTAGGTTCTATCCTAACCGTACCAAATATTT5510.10454277241360992No Hit
GTTTTAGTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAC5460.10359410841711617No Hit
ACATATTGTTGACATCTATTAATAATATCCTCTTTCAAATGAATGTGAGG5400.10245571162132369No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA5320.1009378492269337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)