FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441782
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGA28670.64896261051831No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14210.3216518554400134No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13020.2947154931617857No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT12870.2913201533788158No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT12830.2904147294366905No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12270.27773879424693626No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAC11990.27140082665205917No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11490.2600830273754929No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT10960.24808616014233265No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10780.2440117524027688No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG10600.23993734466320493No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10400.2354102249525784No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC9850.22296064574835553No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA9600.21730174611007239No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8870.20077775916628565No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC8740.19783513135437839No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA8550.19353436762928322No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8510.1926289436871579No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT8380.1896863158752507No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA8120.18380106025143622No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGTAA8120.18380106025143622No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT7990.18085843243952898No Hit
GTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7810.17678402469996513No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT7780.17610495674337115No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7770.17587860075783984No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7430.1681824972497748No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTAGTTT6940.15709105395873982No Hit
ATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTCCATGC6930.1568646979732085No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG6850.15505385008895792No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTAGT6830.15460113811789525No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6810.1541484261468326No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC6640.15030037439280008No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6560.14848952650854946No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA6530.1478104585519555No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6470.14645232263876753No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT6420.1453205427111109No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACA6410.14509418672557958No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATAATTAATCT6380.1444151187689856No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6160.13943528708729644No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6140.1389825751162338No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6100.1380771511741085No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6050.13694537124645187No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5980.1353608793477326No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5950.13468181139113863No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5900.133550031463482No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA5850.13241825153582537No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5800.13128647160816875No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5780.13083375963710608No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTAGTTTGGA5650.12789113182519884No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5640.1276647758396675No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT5640.1276647758396675No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT5600.12675935189754223No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCTAGAATGTTT5570.12608028394094825No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC5510.1247221480277603No Hit
GTGTAAATGTATGTTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5480.12404308007116632No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5470.12381672408563499No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5350.12110045225925908No Hit
CATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5310.1201950283171338No Hit
TCTCGGGGCCGCTTGGGGCTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG5290.11974231634607113No Hit
AATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5290.11974231634607113No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT5250.11883689240394585No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5210.11793146846182054No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5160.1167996885341639No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5140.11634697656310126No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5130.11612062057756993No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA5120.1158942645920386No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5110.11566790860650729No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5100.11544155262097595No Hit
CTCACAGTCAATGAAAAAAGAAGACTGCAATTAATTATGCCTGCTAGGTT5090.11521519663544462No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5070.11476248466438198No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5020.11363070473672536No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA5000.11317799276566269No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT4980.11272528079460005No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC4900.11091443291034946No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4890.11068807692481812No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTTTGTGTA4850.10978265298269282No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCTTTTTTCA4850.10978265298269282No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT4830.10932994101163017No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA4770.10797180509844222No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4760.1077454491129109No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4760.1077454491129109No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT4730.10706638115631692No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT4730.10706638115631692No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC4720.10684002517078559No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAACATACA4650.1052555332720663No Hit
CTCCGAAAGACACCAAATACTCTATAACTGTTTCTCTTCCAAAAGTGAGA4610.10435010932994102No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACACC4570.1034446853878157No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA4570.1034446853878157No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC4560.1032183294022844No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4550.10299197341675306No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA4530.10253926144569042No Hit
GTGTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG4510.10208654947462775No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4510.10208654947462775No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT4510.10208654947462775No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA4480.10140748151803378No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)