FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539429
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28960.5368639802457784No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT19060.3533365836838583No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG16290.30198598888825035No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG15290.28344786802340993No Hit
ATTTATAGGGAGTTTTCGAAAGCATTGTTTCAATTTTTGTACAATATGTT13300.24655700750237752No Hit
TTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA13200.24470319541589347No Hit
GGATATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG12100.224311262464569No Hit
CTCCAAAAGACACCAAATATTCTAGAACAGTTTCTCTTCCAAAAGTAAGA11610.21522758324079724No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA11450.21226148390242275No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11350.2104076718159387No Hit
CCCTATAAATAGGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTC11240.20836847852080628No Hit
ATTAATAGGGAGTTTTCGAAAGCATTGTTTCAATTTTTGTACAATATGTT11200.20762695368621265No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT10800.20021170534027646No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC10240.18983035765596584No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG10210.18927421403002062No Hit
ATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA9390.17407295492085148No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC9240.17129223679112543No Hit
GTAGTAAACCATGTCAATGTTAACATGGGCCTAAAAATCAGACAACTATT8830.16369160723654086No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGCA8650.1603547454808696No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC8490.15738864614249512No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA8260.15312487834358182No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATATCCTCTTTC8140.15090030383980096No Hit
CCTATCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8100.15015877900520735No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT7810.14478272395440364No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT7710.14292891186791962No Hit
GTAAACCATGTCAATGTTAACATGGGCCTAAAAATCAGACAACTATTGTG7540.13977743132089673No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7480.1386651440690063No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAA7410.1373674756084675No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT7380.13681133198252227No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC7150.13254756418360897No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC7100.13162065814036694No Hit
TATTTATAGGGAGTTTTCGAAAGCATTGTTTCAATTTTTGTACAATATGT7050.13069375209712492No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGCATGTTCA7020.13013760847117972No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6980.12939608363658608No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6930.12846917759334406No Hit
GTTGATAATATCCTCTTTCAAATGAATTTGAGGAAAAGAGGGAGTTTGCC6890.12772765275875048No Hit
ATGTTAACATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6740.12494693462902438No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6720.12457617221172759No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6550.12142469166470471No Hit
CCAAAAGACACCAAATATTCTAGAACAGTTTCTCTTCCAAAAGTAAGACA6450.11957087957822068No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAAATTCATTTGAAAG6350.11771706749173663No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT6280.1164193990311978No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA6250.11586325540525261No Hit
AGCGATAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCTAGAA6220.11530711177930737No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAAAGGACAACAGAGT6130.11363868090147175No Hit
AGTCTGTACAACATCTTGAAGCCCTTTTTACCTCTATTACCAATTTTCTT5830.10807724464201962No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA5760.10677957618148079No Hit
CTCTAAGGCCTCCCGATACAGAGCAGATGCGGTGTCGAGGAGATCCCGAA5760.10677957618148079No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCCAAACTAGACATTATT5650.10474038288634833No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5580.10344271442580952No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5560.10307195200851271No Hit
GGAACATATTGTACAAAAATTGAAACAATGCTTTCGAAAACTCCCTATAA5520.10233042717391909No Hit
ACGATGCAGGACCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT5480.10158890233932547No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5460.10121813992202866No Hit
TCCCGATACAGAGCAGATGCGGTGTCGAGGAGATCCCGAACAGACGGAAA5420.10047661508743504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)