FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539429
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28730.5326002124468652No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG20190.37428466026112794No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT17450.32349020909146525No Hit
ATTTATAGGGAGTTTTCGAAAGCATTGTTTCAATTTTTGTACAATATGTT13780.25545530551750095No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG13380.24804005717156472No Hit
TTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA12720.23580489740077007No Hit
CTCCAAAAGACACCAAATATTCTAGAACAGTTTCTCTTCCAAAAGTAAGA12540.2324680356450988No Hit
CCCTATAAATAGGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTC12150.22523816850781106No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA11760.2180083013705233No Hit
GGATATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG11440.21207610269377433No Hit
ATTAATAGGGAGTTTTCGAAAGCATTGTTTCAATTTTTGTACAATATGTT10990.20373394830459618No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10600.19650408116730841No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT10430.1933526006202855No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC9850.18260049051867808No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG9730.18037591601489722No Hit
GTAGTAAACCATGTCAATGTTAACATGGGCCTAAAAATCAGACAACTATT9180.17017994953923501No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC9150.1696238059132898No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATATCCTCTTTC9060.16795537503545416No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA8810.16332084481924405No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT8670.1607255078981664No Hit
ATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA8300.15386640317817543No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGCATGTTCA8190.151827209883043No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGCA8070.14960263537926213No Hit
GTAAACCATGTCAATGTTAACATGGGCCTAAAAATCAGACAACTATTGTG7970.1477488232927781No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7860.14570962999764567No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC7840.14533886758034886No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT7480.1386651440690063No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT7380.13681133198252227No Hit
TATTTATAGGGAGTTTTCGAAAGCATTGTTTCAATTTTTGTACAATATGT7340.13606980714792866No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC7180.13310370780955416No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA7060.13087913330577333No Hit
GTTGATAATATCCTCTTTCAAATGAATTTGAGGAAAAGAGGGAGTTTGCC6930.12846917759334406No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAA6840.12680074671550845No Hit
ATGTTAACATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6540.1212393104560563No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6350.11771706749173663No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6270.1162340178225494No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT6260.116048636613901No Hit
CCTATCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6030.1117848688149877No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAAAGGACAACAGAGT5890.10918953189391005No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA5880.10900415068526165No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAAATTCATTTGAAAG5840.10826262585066802No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA5820.10789186343337122No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5750.10659419497283239No Hit
CCAAAAGACACCAAATATTCTAGAACAGTTTCTCTTCCAAAAGTAAGACA5750.10659419497283239No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5510.10214504596527069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)