FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477419
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22390.46898007829600413No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT15720.32927051499835575No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15170.31775023616571607No Hit
ATTCCTCTTCAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTT14500.30371644195140957No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC13890.29093940542793645No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG13730.28758805158571404No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAATC11650.24402045163682218No Hit
TACTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT11190.23438530934043264No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11180.2341758497252937No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT10970.22977719780737674No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA10510.2201420555109872No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA10270.21511502474765354No Hit
ATCAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAAAG10000.20945961513890313No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9630.20170960937876373No Hit
CACTAAGTATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC9470.19835825553654127No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATACTTAGTGTGGGT9340.19563528053973553No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA9210.1929123055429298No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT9080.19018933054612405No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC9070.18997987093098517No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT9050.18956095170070733No Hit
CAAAACAACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGG9010.18872311324015173No Hit
GTGACGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT8870.18579067862820708No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA8520.17845959209834547No Hit
CCTATTGATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT8320.1742703997955674No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTTTTTTCATTTAC8240.1725947228744562No Hit
ACTGTGGAGTTAGTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG8070.16903390941709484No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA8040.16840553057167815No Hit
GTCTAAGGGCAAATACTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA7570.15856092866014967No Hit
ATTCTATCCTACCCACACTAAGTATTTGCCCTTAGACAAAGGAATTAAAC7440.15583795366334394No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG7390.15479065558764943No Hit
GTTCTTCACAGGGTCCCCAGTCCTCGCGGAGATTGACGAGATGTGAGAGG7330.153533897896816No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA7330.153533897896816No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7220.15122984213028806No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT7180.15039200366973246No Hit
GATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTTGCCATT7070.1480879479032045No Hit
GTGAAGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT6730.1409663209884818No Hit
CTTCAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG6640.1390811844522317No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT6480.13572983061000923No Hit
CCCTTATAAAGAATTTGGAGCTACTGTGGAGTTAGTCTCGTTTTTGCCTT6460.13531091137973145No Hit
CAGTATCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACAATAGTTGC6390.1338446940737591No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA6360.1332163152283424No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6330.1325879363829257No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT6290.13175009792237008No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6270.13133117869209227No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6160.12902712292556434No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6060.1269325267741753No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC5950.12462847100764739No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTGTGGAG5820.12190549601084164No Hit
CTTCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA5760.12064873832000822No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT5730.1200203594745915No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5630.11792576332320247No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG5610.11750684409292467No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC5510.11541224794153564No Hit
GTCTAGAAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5250.10996629794792416No Hit
CTCTAAGGCTTCTCGATACAGAGCTGAGGCGGTGTCTAGAAGATCTCTGA5250.10996629794792416No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT5230.10954737871764633No Hit
GTGCAGAGGTGAATCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5150.1078717017965351No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAAACAACTCTATG5150.1078717017965351No Hit
GTACGGGACCATGCAAAACCTGCACGAGTCCTGCTCAAGGCAACTCTATG5130.1074527825662573No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5120.10724332295111842No Hit
GACTTTGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAACAGTG4970.10410142872403486No Hit
GCACCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGG4950.10368250949375706No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC4930.10326359026347925No Hit
GTTGCCTTGAGCAGGACTCGTGCAGGTTTTGCATGGTCCCGTACTGGTTG4900.10263521141806253No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG4890.10242575180292365No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC4880.10221629218778473No Hit
GAAGATGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4820.1009595344969513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)