FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472980
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA60531.279758129307793No Hit
AATATACACTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG14540.30741257558459134No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC13920.2943041989090448No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT13600.28753858514102076No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13040.2756987610469787No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12620.26681889297644723No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12420.26259038437143223No Hit
ATCCCAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCCTAT12400.2621675335109307No Hit
CACTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12000.2537105163009007No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT11040.23341367499682864No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10120.21396253541375956No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9750.2061397944944818No Hit
GTGCTGGAGAAGGAAAAGGGATGAATAGGCAGAATGCACAGGATTTTTAG9740.20592836906423106No Hit
AGATAAGGGTATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACT9270.19599137384244575No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8850.18711150577191424No Hit
GTTCTATCCCAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAAC8600.18182587001564549No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT8360.17675165968962747No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT7970.1685060679098482No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7810.1651232610258362No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7530.15920334897881516No Hit
GTGCTGGTGTTTGTGTGTATAATTGAATATTATTCAGCTTTTGTAAAGAA7380.1560319675250539No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7280.1539177132225464No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6960.1471520994545224No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTGGGATAGAAC6900.14588354687301788No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6680.14123218740750138No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACACTTACA6640.1403864856864984No Hit
GGATAATAGGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT6620.13996363482599689No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTGGGATAGAACCTAGCAGG6550.1384836568142416No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6330.1338322973487251No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6000.12685525815045035No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5630.11903251723117257No Hit
GTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTCTATCC5630.11903251723117257No Hit
GTATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5500.11628398663791281No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5490.11607256120766206No Hit
CTCACAGTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTT5470.11564971034716057No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5440.11501543405640831No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5390.11395830690515456No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5360.11332403061440231No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG5340.1129011797539008No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT5310.11226690346314856No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC5280.11163262717239629No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT5240.11078692545139329No Hit
GCCTAGCAGCCATGGAAACGACGTATATTTGCGGGAGAGAACAACAGAGT5230.11057550002114254No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA5190.10972979830013954No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACACTTACACC5090.10761554399763204No Hit
CATTGGGATAGAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA5090.10761554399763204No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACA5050.10676984227662903No Hit
GTGCTGGCATCTGCTCAGTTTCTAGGGAGGCCGCAGGCAATTAACAATCA4910.10380988625311853No Hit
GGATAGAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTGAC4780.10106135565985876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)