FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472980
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA59441.2567127574104613No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT13840.2926127954670388No Hit
AATATACACTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG13170.2784472916402385No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13020.27527591018647724No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12430.26280180980168294No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC12270.25941900291767095No Hit
ATCCCAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCCTAT11620.24567634995137214No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT11290.23869931075309736No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT11190.23658505645058986No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11050.23362510042707935No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10820.22876231553131213No Hit
CACTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT10310.2179796185885238No Hit
GTTCTATCCCAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAAC9520.20127700959871453No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8890.18795720749291725No Hit
AGATAAGGGTATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACT8720.1843629751786545No Hit
GTGCTGGAGAAGGAAAAGGGATGAATAGGCAGAATGCACAGGATTTTTAG8680.18351727345765148No Hit
GGATAATAGGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT8620.18224872087614696No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTGGGATAGAAC7690.16258615586282718No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT7380.1560319675250539No Hit
GTGCTGGTGTTTGTGTGTATAATTGAATATTATTCAGCTTTTGTAAAGAA7270.15370628779229567No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7160.1513806080595374No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTGGGATAGAACCTAGCAGG6990.14778637574527465No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6770.14313501627975814No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6700.14165503826800285No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6570.1389065076747431No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6430.13594655165123262No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6390.1351008499302296No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACACTTACA6350.1342551482092266No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG6250.1321408939067191No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6170.13044949046471307No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5830.12326102583618759No Hit
GTATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5780.12220389868493384No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5690.12030106981267706No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA5380.11374688147490379No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5220.11036407459089179No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT5180.10951837286988879No Hit
GTTCTGGATAATAGGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA5170.10930694743963804No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT5140.10867267114888579No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5120.10824982028838429No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5100.10782696942788278No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG5020.10613556598587678No Hit
CATTGGGATAGAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA4930.10423273711362004No Hit
GGATAGAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTGAC4860.10275275910186478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)