FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002488994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75441
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT10411.37988626873981No Hit
ACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACC9871.308307153934863No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG7581.0047586855953659No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG7320.970294667355947No Hit
GCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACCAGCACCGGACCAT7090.9398072666056918No Hit
AAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCT5870.778091488713034No Hit
GAGCAGTAGTCGTGCAGGTTCTGCATGGTCCGGTGCTGGTGGTTGATGAT5770.7648360970824883No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA5570.7383253138213969No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTC5200.6892803647883776No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4560.604445858352885No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG4380.5805861534179028No Hit
GTAAAAGCCTGTTTAGCTTGTATACATGCATACAAAGGCATTAAAGCAGG4200.5567264484829204No Hit
ACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGG3920.5196113519173924No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT3860.5116581169390649No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAAT3860.5116581169390649No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACATACT3800.5037048819607376No Hit
GGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCA3640.48249625535186436No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGT3450.45731101125382745No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA3430.4546599329277184No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC3000.39766174891637174No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA2960.39235959226415346No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG2900.384406357285826No Hit
GAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCAC2770.36717434816611655No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCG2750.36452326984000744No Hit
GTTCAGGAACAGTGAGCCCTGCTCAGAATACTGCCTCTGCCATATCGTCA2720.3605466523508437No Hit
CAATACGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTC2670.3539189565355708No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACCCCCAATTACATATCCCATGAA2660.35259341737251626No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA2590.34331464323113425No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG2580.3419891040680797No Hit
AGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTATTT2520.33403586908975225No Hit
GTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGTACAATATGTT2510.3327103299266977No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT2500.33138479076364313No Hit
GTTTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG2370.3141527816439337No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC2350.3115017033178245No Hit
GGAACATATTGTACAAAAAATCAAATCATGTTTTAGGAAACTTCCTGTAA2210.2929441550350605No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA2190.2902930767089514No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT2170.2876419983828422No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA2160.28631645921978766No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC2150.2849909200567331No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT2130.28233984173062393No Hit
CAAGATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTG2100.2783632242414602No Hit
GAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGC2090.27703768507840565No Hit
CATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGC2090.27703768507840565No Hit
ATGTCATTGGGGGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA2070.2743866067522965No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT2070.2743866067522965No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC2050.27173552842618737No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTA2050.27173552842618737No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC2020.26775891093702364No Hit
GTTTAGCTTGTATACATGCATACAAAGGCATTAAAGCAGGATATCCACAT2010.2664333717739691No Hit
GGAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCA1960.2598056759586962No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG1920.2545035193064779No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC1920.2545035193064779No Hit
GAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGACCCTG1880.24920136265425963No Hit
CAGTTTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAG1870.24787582349120504No Hit
ATTCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCT1860.24655028432815046No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG1860.24655028432815046No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA1860.24655028432815046No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG1830.24257366683898676No Hit
GTCCCCAATCTCCAGTCACTCACCAACTTGTTGTCCTCCAATTTGTCCTG1830.24257366683898676No Hit
GCATAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTT1820.24124812767593218No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC1810.23992258851287762No Hit
GGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATT1810.23992258851287762No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG1740.23064381437149561No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC1690.22401611855622275No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC1690.22401611855622275No Hit
CAATGACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG1670.2213650402301136No Hit
ACTTATCGGGACTGACAATTCTGTCGTGCTGTCCCGCAAGTATACATCGT1580.20943518776262246No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG1530.2028074919473496No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG1520.201481952784295No Hit
GCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTT1510.20015641362124045No Hit
ATGTAATTGGGGGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA1490.19750533529513128No Hit
ATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTGTG1490.19750533529513128No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATA1480.19617979613207673No Hit
CGGTTGTTTCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC1460.19352871780596756No Hit
GCACCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGG1450.192203178642913No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC1450.192203178642913No Hit
CAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTT1420.18822656115374928No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT1400.18557548282764014No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA1400.18557548282764014No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA1370.1815988653384764No Hit
GTCCCAAATCTCCAGTCACTCACCAACTTGTTGTCCTCCAATTTGTCCTG1360.18027332617542186No Hit
GCAGAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCCTATGGCCAAG1350.17894778701236727No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC1320.17497116952320357No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG1310.173645630360149No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG1310.173645630360149No Hit
CATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAA1310.173645630360149No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG1300.1723200911970944No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCATAGACCAGGCC1290.17099455203403985No Hit
GCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGC1280.16966901287098526No Hit
CCAAAACTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTT1280.16966901287098526No Hit
GGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACCAGCAC1280.16966901287098526No Hit
GATTTGGGACTGCGAATTTTGGCCAAGACACACGGGTGTTCCCCCTAGAA1260.16701793454487612No Hit
GGCATAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGT1250.16569239538182157No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG1240.16436685621876698No Hit
GCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGT1220.16171577789265784No Hit
TACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTGTGAG1190.15773916040349412No Hit
CAGTGAGCCCTGCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCG1180.15641362124043956No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG1170.15508808207738498No Hit
CTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACA1160.1537625429143304No Hit
GGGGGCCAAAACTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCA1150.15243700375127583No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT1150.15243700375127583No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA1150.15243700375127583No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT1130.1497859254251667No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC1130.1497859254251667No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGT1100.14580930793600297No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACAACCGTG1100.14580930793600297No Hit
ACAGTTTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACA1090.1444837687729484No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC1090.1444837687729484No Hit
GTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA1080.14315822960989383No Hit
CCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTT1080.14315822960989383No Hit
CCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAG1050.1391816121207301No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCAT1050.1391816121207301No Hit
ATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGA1040.13785607295767555No Hit
ACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACA1040.13785607295767555No Hit
CTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAAC1030.13653053379462096No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTGCATCCT1020.1352049946315664No Hit
GTATGTCAACGAATTGTGGGTCTTTTGGGGTTCGCTGCCCCTTTTACACA960.12725175965323895No Hit
GTACAGTTTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA960.12725175965323895No Hit
CAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTC960.12725175965323895No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA950.1259262204901844No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGG950.1259262204901844No Hit
CCCGCAAGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGG940.12460068132712981No Hit
ACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGG940.12460068132712981No Hit
GTTTCCTAAAACATGATTTGATTTTTTGTACAATATGTTCCTGTGGCAAG940.12460068132712981No Hit
CCTTGCCACAGGAACATATTGTACAAAAAATCAAATCATGTTTTAGGAAA940.12460068132712981No Hit
GTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG940.12460068132712981No Hit
GAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATACCCAAAGA940.12460068132712981No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC930.12327514216407523No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG930.12327514216407523No Hit
CACTTGGCATAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGAT930.12327514216407523No Hit
GACGGAAACTGCACCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAA930.12327514216407523No Hit
GCAGTAGTCGTGCAGGTTCTGCATGGTCCGGTGCTGGTGGTTGATGATCC920.12194960300102066No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGG920.12194960300102066No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA920.12194960300102066No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGC900.11929852467491152No Hit
CCAAAACTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTT900.11929852467491152No Hit
ATCTTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT890.11797298551185695No Hit
GGCCAAAACTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATT890.11797298551185695No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC890.11797298551185695No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAGTTTCCGTCCGTAGGTTT880.11664744634880238No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATT880.11664744634880238No Hit
AATTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATA870.11532190718574781No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN870.11532190718574781No Hit
GCCAAGTCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTT860.11399636802269322No Hit
ATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCGA860.11399636802269322No Hit
CAATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCC850.11267082885963865No Hit
TCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTT840.11134528969658408No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTG840.11134528969658408No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG840.11134528969658408No Hit
ATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAAC830.11001975053352951No Hit
GGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTA830.11001975053352951No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAACAGTATC820.10869421137047494No Hit
TAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTAGCTTGTA820.10869421137047494No Hit
CAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAG820.10869421137047494No Hit
CCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACT820.10869421137047494No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAAGAGAAA820.10869421137047494No Hit
GTGGCAAGGAGCCCCAACCCCCAATTACATATCCCATGAAATTAAGGGAA810.10736867220742037No Hit
GGCCTGTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGTACAAT810.10736867220742037No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC810.10736867220742037No Hit
CTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATGATTTG800.1060431330443658No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCC790.10471759388131123No Hit
TTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTGTGA790.10471759388131123No Hit
GTTTTGTACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTC790.10471759388131123No Hit
CCCCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGT790.10471759388131123No Hit
CCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTAGCTTGTATACATGCA780.10339205471825665No Hit
GGATGTGTCTGCGGCGTTTTATCATCTTCCTCTGCATCCTGCTGCTATGC780.10339205471825665No Hit
ATCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT770.10206651555520208No Hit
ACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTAGCTT760.1007409763921475No Hit
GCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTAGCTTGTATACATGC760.1007409763921475No Hit
GGTTCTGCATGGTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGAC760.1007409763921475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)