FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002488995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75441
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACC7621.010060842247584No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7611.0087353030845296No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG6360.8430429077027081No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA5850.7754404103869249No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5440.7210933047016874No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAAT5330.7065123739080872No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACATACT4920.6521652682228496No Hit
GTAAAAGCCTGTTTAGCTTGTATACATGCATACAAAGGCATTAAAGCAGG4760.6309566416139765No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG4520.5991437017006668No Hit
GCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACCAGCACCGGACCAT4370.5792606142548481No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTC4310.5713073792765208No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG4290.5686563009504115No Hit
GAGCAGTAGTCGTGCAGGTTCTGCATGGTCCGGTGCTGGTGGTTGATGAT4270.5660052226243024No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC3770.4997282644715738No Hit
ACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGG3550.47056640288437324No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT3330.44140454129717266No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTA3300.4374279238080089No Hit
GTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGTACAATATGTT3190.42284699301440865No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG3130.41489375803608114No Hit
ATTCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCT3070.4069405230577537No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGT3010.39898728807942635No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA3010.39898728807942635No Hit
GTTTAGCTTGTATACATGCATACAAAGGCATTAAAGCAGGATATCCACAT3000.39766174891637174No Hit
GGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCA2830.375127583144444No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA2800.3711509656552803No Hit
AAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCT2800.3711509656552803No Hit
GTTTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG2780.36849988732917116No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACCCCCAATTACATATCCCATGAA2750.36452326984000744No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG2740.3631977306769528No Hit
GTTCAGGAACAGTGAGCCCTGCTCAGAATACTGCCTCTGCCATATCGTCA2660.35259341737251626No Hit
CAATACGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTC2630.34861679988335253No Hit
GAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCAC2560.33933802574197053No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA2460.3260826341114248No Hit
CAAGATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTG2450.32475709494837024No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT2440.3234315557853157No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC2360.3128272424808791No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA2300.30487400750255167No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA2300.30487400750255167No Hit
ATGTCATTGGGGGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA2240.2969207725242242No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCG2210.2929441550350605No Hit
GGAACATATTGTACAAAAAATCAAATCATGTTTTAGGAAACTTCCTGTAA2210.2929441550350605No Hit
GTCCCCAATCTCCAGTCACTCACCAACTTGTTGTCCTCCAATTTGTCCTG2140.2836653808936785No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC2090.27703768507840565No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC2060.2730610675892419No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC2030.2690844501000782No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT1890.2505269018173142No Hit
GCATAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTT1880.24920136265425963No Hit
GGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATT1870.24787582349120504No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA1850.2452247451650959No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC1830.24257366683898676No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT1820.24124812767593218No Hit
GTCCCAAATCTCCAGTCACTCACCAACTTGTTGTCCTCCAATTTGTCCTG1810.23992258851287762No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCATAGACCAGGCC1770.2346204318606593No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG1750.23196935353455017No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC1730.22931827520844103No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT1710.2266671968823319No Hit
GGAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCA1680.22269057939316816No Hit
CATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGC1680.22269057939316816No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATA1680.22269057939316816No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC1650.21871396190400444No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG1630.2160628835778953No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA1630.2160628835778953No Hit
AGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTATTT1630.2160628835778953No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT1610.21341180525178616No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG1570.20810964859956788No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG1560.2067841094365133No Hit
GAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGACCCTG1560.2067841094365133No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG1520.201481952784295No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTGCATCCT1510.20015641362124045No Hit
ATGTAATTGGGGGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA1510.20015641362124045No Hit
GGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACCAGCAC1500.19883087445818587No Hit
CAGTTTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAG1440.19087763947985842No Hit
CAATGACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG1420.18822656115374928No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG1370.1815988653384764No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC1370.1815988653384764No Hit
GCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTT1360.18027332617542186No Hit
GATTTGGGACTGCGAATTTTGGCCAAGACACACGGGTGTTCCCCCTAGAA1310.173645630360149No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC1300.1723200911970944No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA1300.1723200911970944No Hit
CAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTT1300.1723200911970944No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC1280.16966901287098526No Hit
CCAAAACTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTT1260.16701793454487612No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC1240.16436685621876698No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC1230.1630413170557124No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGT1210.16039023872960326No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG1210.16039023872960326No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACAACCGTG1210.16039023872960326No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCC1190.15773916040349412No Hit
CGGTTGTTTCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC1190.15773916040349412No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG1190.15773916040349412No Hit
CAGTGAGCCCTGCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCG1180.15641362124043956No Hit
GTTTCCTAAAACATGATTTGATTTTTTGTACAATATGTTCCTGTGGCAAG1180.15641362124043956No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA1180.15641362124043956No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT1170.15508808207738498No Hit
GTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA1170.15508808207738498No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG1160.1537625429143304No Hit
GTACAGTTTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA1150.15243700375127583No Hit
GGCCAAAACTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATT1140.15111146458822125No Hit
GCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGC1120.1484603862621121No Hit
CATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAA1120.1484603862621121No Hit
GGCATAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGT1110.14713484709905755No Hit
ACGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAA1100.14580930793600297No Hit
ATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTGTG1100.14580930793600297No Hit
ATTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC1090.1444837687729484No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA1090.1444837687729484No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG1080.14315822960989383No Hit
TCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTT1080.14315822960989383No Hit
ACTTATCGGGACTGACAATTCTGTCGTGCTGTCCCGCAAGTATACATCGT1060.1405071512837847No Hit
GCAGAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCCTATGGCCAAG1060.1405071512837847No Hit
GAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGC1050.1391816121207301No Hit
AATTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATA1040.13785607295767555No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT1030.13653053379462096No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG1030.13653053379462096No Hit
ACAGTTTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACA1020.1352049946315664No Hit
GTGGCAAGGAGCCCCAACCCCCAATTACATATCCCATGAAATTAAGGGAA1020.1352049946315664No Hit
CCAAAACTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTT1020.1352049946315664No Hit
GTATGTCAACGAATTGTGGGTCTTTTGGGGTTCGCTGCCCCTTTTACACA1010.13387945546851182No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA1010.13387945546851182No Hit
GCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGT1000.13255391630545724No Hit
GCCAAGTCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTT980.1299028379793481No Hit
GCACCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGG980.1299028379793481No Hit
GTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAA970.12857729881629354No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA970.12857729881629354No Hit
CCCCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGT960.12725175965323895No Hit
CCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTT940.12460068132712981No Hit
GAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATACCCAAAGA940.12460068132712981No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG910.12062406383796609No Hit
CTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATGATTTG910.12062406383796609No Hit
GATCGGCAGAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCCTATGG910.12062406383796609No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGC900.11929852467491152No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG900.11929852467491152No Hit
CTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAAC900.11929852467491152No Hit
CTCGAAGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATCAGGAC890.11797298551185695No Hit
ACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACA890.11797298551185695No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAGTTTCCGTCCGTAGGTTT890.11797298551185695No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTG890.11797298551185695No Hit
GGGGGCCAAAACTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCA880.11664744634880238No Hit
TAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTAGCTTGTA880.11664744634880238No Hit
GGCCAAGTCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATT870.11532190718574781No Hit
ACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGG860.11399636802269322No Hit
CCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAG860.11399636802269322No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCAT860.11399636802269322No Hit
ATCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT850.11267082885963865No Hit
GCTTGTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGT850.11267082885963865No Hit
GCCCTGCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACT820.10869421137047494No Hit
GAGCAACGGGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAA820.10869421137047494No Hit
TACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTGTGAG810.10736867220742037No Hit
ATCTTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT810.10736867220742037No Hit
GGCCTGTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGTACAAT810.10736867220742037No Hit
GCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGACCCTGTACCGAA810.10736867220742037No Hit
CCTTGCCACAGGAACATATTGTACAAAAAATCAAATCATGTTTTAGGAAA810.10736867220742037No Hit
GTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG810.10736867220742037No Hit
AACGTATTGTGGGTCTTTTGGGGTTCGCTGCCCCTTTTACACAATGTGGA800.1060431330443658No Hit
GGTTCTGCATGGTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGAC800.1060431330443658No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGG790.10471759388131123No Hit
ATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAAC790.10471759388131123No Hit
GCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTAGCTTGTATACATGC790.10471759388131123No Hit
TCGTAGGGCTTTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGG780.10339205471825665No Hit
CACTTGGCATAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGAT780.10339205471825665No Hit
CCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACT780.10339205471825665No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN780.10339205471825665No Hit
GCCCCAACCCCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATC780.10339205471825665No Hit
CTCAAGATGTTGTACAGTTTTGGCCCCCAATACCACATCATCCATATAAC770.10206651555520208No Hit
CTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACA770.10206651555520208No Hit
CAATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCC770.10206651555520208No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAAGAGAAA760.1007409763921475No Hit
GGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTA760.1007409763921475No Hit
GGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAA760.1007409763921475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)