FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460561
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35250.7653709280638178No Hit
ATTAACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT12130.26337444985571945No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG9260.20105914308853767No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC8890.19302546242517277No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT8600.18672879379713003No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATTCATGTAT8170.17739235410727353No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7330.15915372773639105No Hit
CATTTAAACCCTACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7110.15437694463925516No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG6980.1515543000818567No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6720.14590901096705974No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6660.14460625194056814No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6550.14221786039200018No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6440.13982946884343225No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6410.13917808933018647No Hit
CTCAATAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6240.1354869387551269No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6080.13201291468448262No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA5830.12658475207410094No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAGATC5820.12636762556968567No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG5790.12571624605643988No Hit
GCATGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG5690.12354498101228718No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5200.11290578229593909No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA5160.11203727627827802No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5060.10986601123412534No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA4790.10400359561491311No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG4770.10356934260608258No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4760.10335221610166731No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)