FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460561
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35210.7645024220461568No Hit
ATTAACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT11160.24231317892743848No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG10370.22516018507863236No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT8670.1882486793280369No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATTCATGTAT8250.17912936614259564No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7520.16327913132028113No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7250.1574167157010689No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6750.14656039048030553No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6750.14656039048030553No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC6670.1448233784449834No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA6630.14395487242732233No Hit
CTCAATAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6560.14243498689641546No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6540.14200073388758494No Hit
CATTTAAACCCTACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6520.1415664808787544No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG6510.14134935437433913No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6380.13852670981694062No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAGATC6370.13830958331252535No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG6140.13331567371097422No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6140.13331567371097422No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA5440.1181168184019055No Hit
GCATGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG5410.11746543888865969No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA5260.11420854132243069No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG4750.10313508959725204No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4740.10291796309283678No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4610.10009531853543831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)