FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496149
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32470.6544405007366739No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG30440.6135253724183662No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATACCCCATGAA23970.48312099792602625No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT22260.44865554500764887No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA19400.3910115711207722No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT19380.39060846640827657No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC19210.3871820763520636No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG18600.3748873826209465No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA18110.3650113171648033No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA16900.340623482058817No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTTTTGTC16690.3363908825776128No Hit
ATTACATACCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG16420.3309489689589216No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT15480.3120030474716265No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT15470.31180149511537864No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAAGTAGAGGACAAACGGGCAAC15350.30938286684040484No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTT14960.30152232494673975No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA14070.28358416524068375No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG13480.27169257622206233No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG13420.2704832620845754No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG13010.26221961547841477No Hit
CCTTTATGCCGCTGTTACCCATTTTCTTTTGTCTTTGGGTATACATTTAA12940.26080874898468No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC12630.25456062594099754No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC12510.2521419976660237No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATCATCAACAACC12100.24387835105986305No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC12100.24387835105986305No Hit
CCCAATTACATACCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT11910.24004885629115447No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT11860.23904109450991537No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG11850.23883954215366757No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATGGGTAACAGCGGCATAAA11680.2354131520974546No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATGGGTAACAGCGGCATAA11660.23501004738495898No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG11180.2253355342850636No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG11120.22412622014757666No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC10800.21767654474764636No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA10770.21707188767890293No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA10580.2132423929101943No Hit
CAATTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTG10510.21183152641645955No Hit
CCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTA10390.20941289814148573No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA10230.2061880604415206No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA10190.2053818510165293No Hit
CAATTACATACCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG10180.20518029866028148No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG10160.20477719394778587No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG10110.20376943216654672No Hit
AGACTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTT10050.20256011802905982No Hit
CCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGCCAAG9920.19993993739783814No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA9920.19993993739783814No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA9890.19933528032909467No Hit
AGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATA9770.19691665205412082No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT9690.19530423320413828No Hit
GTTCAGGAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCA9540.19228094786042096No Hit
CCCTTAACTTCATGGGGTATGTAATTGGGAGTTGGGGCACATTGCCACAG9420.1898623195854471No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT9410.1896607672291993No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT9380.18905611016045584No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTTTTG9380.18905611016045584No Hit
GTATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAA9340.18824990073546455No Hit
AGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTATTT9280.18704058659797762No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT9140.18421885361050813No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT8990.1811955682667908No Hit
GACAATGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAAC8980.180994015910543No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG8920.17978470177305608No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATACCCC8780.17696296878558657No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC8750.17635831171684313No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG8690.1751489975793562No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATC8520.17172260752314325No Hit
GTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT8510.17152105516689542No Hit
GTACAGTCTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA8490.1711179504543998No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC8450.1703117410294085No Hit
CTAGTAAATTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT8270.16668379861694774No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT8180.16486982741071735No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT8010.1614434373545044No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTGTC7810.15741239022954798No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG7790.15700928551705234No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCAGGCC7670.1545906572420785No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAA7600.15317979074834376No Hit
CATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAA7280.1467301153484135No Hit
CACTTGGCACAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGAT7220.14552080121092656No Hit
TTACATACCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGA7210.14531924885467873No Hit
GCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT7190.1449161441421831No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT7040.14189285879846578No Hit
GCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGC6890.13886957345474849No Hit
GCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAG6870.13846646874225282No Hit
ATGTTGTACAGTCTTGGCCCCCAAAACCACATCATCCATATAACTGAAAG6660.13423386926104858No Hit
GTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA6460.1302028221360922No Hit
CAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTC6360.12818729857361397No Hit
GTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTATTTT6350.12798574621736614No Hit
CTTCATGGGGTATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATA6340.12778419386111833No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTGCATCCT6240.12576867029864014No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6120.12335004202366627No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAGTTTCCGTCCGTAGGTTT6120.12335004202366627No Hit
CCCAAAGACAAAAGAAAATGGGTAACAGCGGCATAAAGGGACTCAAGATG6100.12294693731117064No Hit
CTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTG6090.12274538495492282No Hit
CCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACT6070.12234228024242719No Hit
GGCCAAGACTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATT6040.1217376231736837No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC5930.11952054725495768No Hit
GGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAA5930.11952054725495768No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGACTGTACAACATCTTGAG5870.11831123311747074No Hit
GGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTA5830.11750502369247949No Hit
ATCTTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT5830.11750502369247949No Hit
GTCTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGACAATG5780.11649726191124037No Hit
TAGCAGCAGGATGCAGAGGAAGATGATAAAACGCCGCAGACACATCCAGC5760.11609415719874475No Hit
TACATACCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAG5760.11609415719874475No Hit
CCATATAACTGAAAGCCAGACAATGGGGGAAAGCCCTACGAACCACTGAA5740.11569105248624909No Hit
ATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAAACAGG5740.11569105248624909No Hit
ATGCAGAGGAAGATGATAAAACGCCGCAGACACATCCAGCGATAACCAGG5690.11468329070500999No Hit
CTCACCAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC5680.11448173834876217No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5680.11448173834876217No Hit
GTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACACG5670.11428018599251434No Hit
CAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCG5640.11367552892377088No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGACTGTACAACATCTTG5640.11367552892377088No Hit
CCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAAC5620.11327242421127524No Hit
CTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACA5570.11226466243003613No Hit
CCCATTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGCCAAG5480.11045069122380576No Hit
ATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACA5440.10964448179881447No Hit
ATATAACTGAAAGCCAGACAATGGGGGAAAGCCCTACGAACCACTGAACA5400.10883827237382318No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTT5360.1080320629488319No Hit
CTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACT5350.10783051059258408No Hit
CTCAATTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCAT5300.10682274881134497No Hit
TCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTT5260.10601653938635369No Hit
TGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATG5250.10581498703010589No Hit
CATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGC5250.10581498703010589No Hit
GCATAAAGGGACTCAAGATGTTGTACAGTCTTGGCCCCCAAAACCACATC5210.1050087776051146No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAATTTACTAGTG5190.10460567289261895No Hit
CTCTACTTCCAGGATCATCAACAACCAGCACCGGACCATGCAAAACCTGC5140.10359791111137985No Hit
ATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCAA5130.10339635875513202No Hit
GTCCCAAATCTCCAGTCACTCACCAACCTGTTGTCCTCCAATTTGTCCTG5090.10259014933014074No Hit
ATCGAAGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATCAGGAC5080.10238859697389294No Hit
CTGTTACCCATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAA5070.1021870446176451No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG5040.10158238754890164No Hit
ATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATACCCAAAGACAAA5000.10077617812391036No Hit
GCGATAACCAGGACAAATTGGAGGACAACAGGTTGGTGAGTGACTGGAGA5000.10077617812391036No Hit
CATCCAGCGATAACCAGGACAAATTGGAGGACAACAGGTTGGTGAGTGAC4990.10057462576766253No Hit
TATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCAAA4980.1003730734114147No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)