FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496149
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG34250.6903168201487859No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32400.6530296342429391No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATACCCCATGAA25400.5119429848694645No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT22860.46074868638251815No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT20290.40894973082682823No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTTTTGTC20180.40673265490810223No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC19940.40189539835815447No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA19780.39867056065818934No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG18600.3748873826209465No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA18520.373274963770964No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTT18140.3656159742335468No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA17120.3450576338962691No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAAGTAGAGGACAAACGGGCAAC15830.3190573799403002No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT15560.313615466321609No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT15390.3101890762653961No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA14980.3019254296592354No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG14390.290033840640614No Hit
CCTTTATGCCGCTGTTACCCATTTTCTTTTGTCTTTGGGTATACATTTAA14170.28559968880316194No Hit
ATTACATACCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG13980.28177019403445336No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG13820.27854535633448824No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG13030.26262272019091043No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC13030.26262272019091043No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC13010.26221961547841477No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG12300.24790939818481947No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATGGGTAACAGCGGCATAA12010.24206437985363266No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG11790.23763022801618064No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG11740.2366224662349415No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA11570.23319607617872856No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG11540.2325914191099851No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATGGGTAACAGCGGCATAAA11500.23178520968499383No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC11470.23118055261625034No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT11420.23017279083501124No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATCATCAACAACC11230.22634329606630266No Hit
CCCAATTACATACCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT11200.2257386389975592No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA11180.2253355342850636No Hit
GTTCAGGAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCA11120.22412622014757666No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA10950.22069983009136368No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA10890.21949051595387675No Hit
AGACTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTT10880.21928896359762895No Hit
CCCTTAACTTCATGGGGTATGTAATTGGGAGTTGGGGCACATTGCCACAG10850.21868430652888546No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA10840.21848275417263768No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG10830.21828120181638985No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATACCCC10590.21344394526644211No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT10590.21344394526644211No Hit
CAATTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTG10500.21162997406021178No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA10480.21122686934771612No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT10450.21062221227897263No Hit
CCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTA10420.2100175552102292No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTTTTG10380.2092113457852379No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG10060.20276167038530765No Hit
CCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGCCAAG9890.19933528032909467No Hit
GTATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAA9860.19873062326035124No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT9770.19691665205412082No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC9750.1965135473416252No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC9740.19631199498537738No Hit
CAATTACATACCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG9570.19288560492916443No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT9550.1924825002166688No Hit
GTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT9430.19006387194169494No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG9130.1840173012542603No Hit
AGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATA9110.18361419654176467No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT9090.18321109182926903No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT9070.1828079871167734No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT8840.17817228292307352No Hit
AGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTATTT8810.17756762585433006No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG8710.17555210229185184No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCAGGCC8600.1733350263731258No Hit
GTACAGTCTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA8550.17232726459188671No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATC8520.17172260752314325No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTGTC8320.16769156039818683No Hit
GACAATGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAAC8290.16708690332944337No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT8180.16486982741071735No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC8170.16466827505446952No Hit
GCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT7790.15700928551705234No Hit
CTAGTAAATTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7780.1568077331608045No Hit
CAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTC7440.1499549530483786No Hit
GCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGC7350.14814098184214822No Hit
CATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAA7330.14773787712965258No Hit
CACTTGGCACAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGAT7160.14431148707343963No Hit
CCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACT7110.14330372529220053No Hit
CTTCATGGGGTATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATA7090.1429006205797049No Hit
ATGTTGTACAGTCTTGGCCCCCAAAACCACATCATCCATATAACTGAAAG7060.14229596351096144No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTGCATCCT7010.1412882017297223No Hit
GTCTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGACAATG7000.14108664937347448No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAA6970.14048199230473102No Hit
GTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA6890.13886957345474849No Hit
GGCCAAGACTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATT6860.13826491638600502No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGACTGTACAACATCTTGAG6670.1344354216172964No Hit
CCCAAAGACAAAAGAAAATGGGTAACAGCGGCATAAAGGGACTCAAGATG6670.1344354216172964No Hit
GCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAG6630.13362921219230514No Hit
TTACATACCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGA6590.13282300276731385No Hit
ATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAAACAGG6510.13121058391733129No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAGTTTCCGTCCGTAGGTTT6510.13121058391733129No Hit
CTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTG6480.13060592684858782No Hit
CTCACCAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC6410.1291950603548531No Hit
CCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAAC6410.1291950603548531No Hit
CTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACT6240.12576867029864014No Hit
CAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCG6120.12335004202366627No Hit
GTCCCAAATCTCCAGTCACTCACCAACCTGTTGTCCTCCAATTTGTCCTG6040.1217376231736837No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC5940.1197220996112055No Hit
GTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACACG5920.11931899489870987No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGACTGTACAACATCTTG5880.11851278547371857No Hit
GGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTA5870.11831123311747074No Hit
CCCATTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGCCAAG5870.11831123311747074No Hit
CCATATAACTGAAAGCCAGACAATGGGGGAAAGCCCTACGAACCACTGAA5850.11790812840497512No Hit
ATGCAGAGGAAGATGATAAAACGCCGCAGACACATCCAGCGATAACCAGG5810.11710191897998383No Hit
GGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAA5740.11569105248624909No Hit
ATATAACTGAAAGCCAGACAATGGGGGAAAGCCCTACGAACCACTGAACA5700.1148848430612578No Hit
CTCTACTTCCAGGATCATCAACAACCAGCACCGGACCATGCAAAACCTGC5590.1126677671425318No Hit
GTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTATTTT5520.11125690064879704No Hit
GGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGGAAGTAGAGGAC5510.11105534829254922No Hit
ATCTTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT5500.11085379593630139No Hit
ATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACA5490.11065224358005359No Hit
TAGCAGCAGGATGCAGAGGAAGATGATAAAACGCCGCAGACACATCCAGC5470.11024913886755792No Hit
CATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGC5460.11004758651131011No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTT5430.10944292944256664No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5420.10924137708631884No Hit
GCATAAAGGGACTCAAGATGTTGTACAGTCTTGGCCCCCAAAACCACATC5400.10883827237382318No Hit
CTCAATTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCAT5360.1080320629488319No Hit
TACATACCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAG5340.10762895823633627No Hit
TCGTAGGGCTTTCCCCCATTGTCTGGCTTTCAGTTATATGGATGATGTGG5320.10722585352384063No Hit
TCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTT5310.1070243011675928No Hit
CTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACA5280.10641964409884934No Hit
ATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATACCCAAAGACAAA5280.10641964409884934No Hit
CTTTCACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA5220.1052103299613624No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACA5130.10339635875513202No Hit
GTAAAAGCCTGTTTTGCTTGTATACATGCATATAAAGGCATTAAAGCAGG5110.10299325404263639No Hit
AAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCT5110.10299325404263639No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5070.1021870446176451No Hit
GTTTTGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT5040.10158238754890164No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA4990.10057462576766253No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)