FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396304
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12440.3139004400662118No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC11590.29245225887197707No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11170.2818543340465905No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA11040.2785740239815899No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10650.2687330937865881No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9580.2417336186361985No Hit
CTCCAAAAGACACCAAATACTCTAGAACAACTTCTCTTCCAAAAGTAAGA9420.23769631394081311No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT8540.21549113811619364No Hit
CTCTAGAACAACTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA8470.21372481731196255No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT8150.20565020792119182No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC7860.19833259316080581No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG7500.18924865759618878No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAACTCAGACAAATTTT7090.17890306431426378No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC7010.17688441196657112No Hit
ATTTATAGGAAGTTTGCGAAAACATTGTTTCAATTTTTGTACAATATGTT6990.17637974887964794No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6900.17410876498849367No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG6880.1736041019015705No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT6880.1736041019015705No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6590.16628648714118455No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6490.16376317170656868No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG6420.1619968509023376No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA6340.1599781985546449No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA6310.15922120392426015No Hit
ATGTTAACATGGGCCTAAAACTCAGACAAATTTTGTGGTTTCACATTTCC6010.15165125762041262No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA5990.15114659453348944No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5950.15013726835964308No Hit
TCACCATACAGCACTAAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGA5890.1486232790988736No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGCAAACTTCCTATAA5890.1486232790988736No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG5780.14584763212079616No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5610.1415579958819492No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC5590.14105333279502602No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5460.13777302273002542No Hit
GCACTAAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5440.13726835964310227No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA5440.13726835964310227No Hit
ATGATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG5330.13449271266502483No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG5290.1334833864911785No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG5230.13196939723040896No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGAACGTCCTTTGTCTA5210.1314647341434858No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT5110.12894141870886996No Hit
GCTTACTACTAAATCCCTGGATGCTTGGTCTTCCAAATTACTTCCCACCC5060.12767976099156203No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5060.12767976099156203No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC5020.1266704348177157No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCAAGAAGATTGACGATATGG5010.12641810327425412No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC4970.12540877710040776No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCTGC4910.12389478783963825No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4910.12389478783963825No Hit
ATACAGCACTAAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGAATCTG4860.12263313012233032No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG4820.121623803948484No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC4680.11809116234002179No Hit
GTCCAAGGGATACTAACATTGAGATCCCCGAGATTGAGATCTTCTGCAAC4540.1145585207315596No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA4530.11430618918809803No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4530.11430618918809803No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4430.11178287375348218No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4370.11026888449271266No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4350.1097642214057895No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC4290.10825023214502No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4270.10774556905809682No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4250.10724090597117365No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC4180.10547458516694255No Hit
TGTTAACATTGACATAGCTTACTACTAAATCCCTGGATGCTTGGTCTTCC4140.10446525899309621No Hit
GGCCTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACGGGGCG4130.10421292744963462No Hit
CTCTAAGGCCTCCCGAAACAGAGCAGAGGCGGTGTCGAGGAGATCCCGAA4120.10396059590617303No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4110.10370826436271144No Hit
GCCTTAGAGTCTCCGGAACATGGTACACCTCACCATACAGCACTAAGGCA4090.10320360127578829No Hit
GATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC4050.10219427510194194No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3990.10068028584117243No Hit
GGCCTATACTTTCCTGCTGGTGGCTCAAGTTCCGGAACAGTAAACCCTGT3970.10017562275424925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)