FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613254
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51310.836684310253174No Hit
CATTAATAATGCCTGCTAGGTTCTATCCCAATAGTACCAAATATTTGCCC22620.36885205803794185No Hit
GTATGTAAATAGTGTCTAGTTTTGAAGTAATGATTAACTGCATGGTCAGG20290.3308580131560495No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACTATT19510.3181389766719826No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAGTGTCTAGTTT16690.2721547678449713No Hit
ATGTAAATAGTGTCTAGTTTTGAAGTAATGATTAACTGCATGGTCAGGAT13930.22714894643981123No Hit
CTCCAAAAGACACCAAATACTCAAGTACAGTTTCTCTTCCAAAAGTAAGA13820.2253552361664172No Hit
ATGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCC13480.21981104077592645No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12930.21084248940895617No Hit
GGGACTTAGTTGTCAGTTATGTCAATGTTAATATGGGCCTAAAAATTAGA11850.19323151581563267No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATGCGTTGACAAACC11780.19209006382347282No Hit
TTATCAATGATAATCTCCTTTTCTCATTTACTGTAAGAGGGCCTACATAT11310.18442602901897093No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA10860.17708812335508617No Hit
GGATCATATTGTACTAAAAATCAAGCAATGTTTTCGAAAACTTCCTGTTA10830.1765989296441605No Hit
ATCAATAGGTGTCAACAATATGTAGGCCCTCTTACAGTAAATGAGAAAAG10770.1756205422223092No Hit
ATATGATCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA10460.17056554054274412No Hit
TTATTATCCTGACCATGCAGTTAATCATTACTTCAAAACTAGACACTATT10310.16811957198811586No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAGTGTCTAGT10220.1666519908553389No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGTG9900.16143392460546527No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9760.15915102062114556No Hit
GGGAATTAGTTGTCAGTTATGTCAATGTTAATATGGGCCTAAAAATTAGA9360.15262843780880353No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9100.14838875898078122No Hit
CATTGATAATGCCTGCTAGGTTCTATCCCAATAGTACCAAATATTTGCCC9020.1470842424183128No Hit
GTTTTATGAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAC8990.14659504870738715No Hit
GTTCTATCCCAATAGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC8970.14626891956677004No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG8890.14496440300430163No Hit
GAGTATGTAAATAGTGTCTAGTTTTGAAGTAATGATTAACTGCATGGTCA8830.14398601558245033No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA8740.14251843444967338No Hit
CTCAAGTACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA8520.13893101390288526No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCCGACATTCATTTGAAGG8440.13762649734041685No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8200.13371294765301164No Hit
CCTATTGATAATGTCCTCCTTCAAATGAATGTCGGGAAAGGAGGGAGTTT7820.12751649398128673No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCCGAC7610.12409213800480715No Hit
CATTTGAACCCTCATAAAACCAAGCGATGGGGCTACTCCCTTAACTTCAT7230.11789568433308222No Hit
ATCCCAATAGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT7170.1169172969112309No Hit
ACATATTGTTGACACCTATTGATAATGTCCTCCTTCAAATGAATGTCGGG7130.11626503862999671No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7130.11626503862999671No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7040.11479745749721974No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7020.11447132835660265No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6920.11284068265351714No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6920.11284068265351714No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6880.11218842437228294No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTACT6850.11169923066135728No Hit
CTCTAAGGCCTCCCGATACAGAGCCGAGGCGGTGTCGAGGAGATCTCGAA6820.11121003695043162No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6780.11055777866919743No Hit
GTCTATTAACAGGAAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA6760.11023164952858033No Hit
CCTTACCACAGGATCATATTGTACTAAAAATCAAGCAATGTTTTCGAAAA6750.11006858495827176No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6750.11006858495827176No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6590.10745955183333496No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6570.10713342269271786No Hit
GTTGTCAATATGCCCTGAGCCTGCGGGCTCCACCCCAAAAGACCTCCGTG6530.10648116441148367No Hit
GTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTATTATCCTGAC6430.10485051870839815No Hit
GGGTAAAGGTTCAGATATTGTTTGCACAGAAAGGCCTTGTAAGTTGGCGA6420.1046874541380896No Hit
AGTTAAGGGAGTAGCCCCATCGCTTGGTTTTATGAGGGTTCAAATGTATA6410.10452438956778105No Hit
CTGTAAGAGGGCCTACATATTGTTGACACCTATTGATAATGTCCTCCTTC6410.10452438956778105No Hit
TCCACAGAGTATGTAAATAGTGTCTAGTTTTGAAGTAATGATTAACTGCA6350.10354600214592974No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6270.10224148558346134No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6250.10191535644284425No Hit
TGTAAATAGTGTCTAGTTTTGAAGTAATGATTAACTGCATGGTCAGGATA6240.10175229187253569No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6230.10158922730222712No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6170.10061083988037584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)