FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613254
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51070.8327707605657689No Hit
GTATGTAAATAGTGTCTAGTTTTGAAGTAATGATTAACTGCATGGTCAGG22330.3641231854989939No Hit
CATTAATAATGCCTGCTAGGTTCTATCCCAATAGTACCAAATATTTGCCC19460.3173236538204398No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACTATT18890.30802897331285245No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAGTGTCTAGTTT16790.2737854135480568No Hit
CTCCAAAAGACACCAAATACTCAAGTACAGTTTCTCTTCCAAAAGTAAGA14740.24035717663480385No Hit
ATGTAAATAGTGTCTAGTTTTGAAGTAATGATTAACTGCATGGTCAGGAT13070.21312539339327588No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATGCGTTGACAAACC12500.2038307128856885No Hit
ATGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCC11920.19437296780779253No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAGTGTCTAGT11380.1855674810111308No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGTG10610.17301150909737237No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10480.1708916696833612No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA10300.1679565074178073No Hit
ATATGATCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA10270.16746731370688167No Hit
GGGACTTAGTTGTCAGTTATGTCAATGTTAATATGGGCCTAAAAATTAGA9970.16257537659762514No Hit
ATCAATAGGTGTCAACAATATGTAGGCCCTCTTACAGTAAATGAGAAAAG9740.15882489148052847No Hit
GGATCATATTGTACTAAAAATCAAGCAATGTTTTCGAAAACTTCCTGTTA9600.15654198749620876No Hit
TTATCAATGATAATCTCCTTTTCTCATTTACTGTAAGAGGGCCTACATAT9550.155726664644666No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8860.14447520929337598No Hit
CTCAAGTACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA8830.14398601558245033No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA8710.14202924073874773No Hit
CATTGATAATGCCTGCTAGGTTCTATCCCAATAGTACCAAATATTTGCCC8700.1418661761684392No Hit
GAGTATGTAAATAGTGTCTAGTTTTGAAGTAATGATTAACTGCATGGTCA8660.14121391788720497No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8490.1384418201919596No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCCGACATTCATTTGAAGG8400.13697423905918266No Hit
TTATTATCCTGACCATGCAGTTAATCATTACTTCAAAACTAGACACTATT8390.1368111744888741No Hit
GTTCTATCCCAATAGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC8360.13632198077794844No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG8320.13566972249671427No Hit
GTTTTATGAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAC8010.13061472081714917No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCCGAC7600.12392907343449859No Hit
GGGAATTAGTTGTCAGTTATGTCAATGTTAATATGGGCCTAAAAATTAGA7550.12311375058295583No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7520.1226245568720302No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA7370.12017858831740193No Hit
CCTATTGATAATGTCCTCCTTCAAATGAATGTCGGGAAAGGAGGGAGTTT7320.11936326546585917No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7320.11936326546585917No Hit
GTCTATTAACAGGAAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA7190.11724342605184801No Hit
CATTTGAACCCTCATAAAACCAAGCGATGGGGCTACTCCCTTAACTTCAT7060.11512358663783685No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6860.11186229523166584No Hit
CTCTAAGGCCTCCCGATACAGAGCCGAGGCGGTGTCGAGGAGATCTCGAA6810.11104697238012308No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6810.11104697238012308No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6680.10892713296611194No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6540.10664422898179221No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTACT6390.10419826042716396No Hit
ATCCCAATAGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT6380.1040351958568554No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6320.1030568084350041No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6270.10224148558346134No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6260.10207842101315279No Hit
ACATATTGTTGACACCTATTGATAATGTCCTCCTTCAAATGAATGTCGGG6140.10012164616945017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)