FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483654
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCA15790.3264730571855087No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA12900.266719597067325No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG12460.2576221844541759No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12130.2507991249943141No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11710.24211523113630817No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT10950.22640151844086887No Hit
CCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCT10390.21482299329686097No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10350.2139959557865747No Hit
GTATACAGGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT10090.2086202119697139No Hit
GTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCA9890.2044850244182825No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT9610.19869576184627855No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGG9600.19848900246870696No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA9510.19662816807056285No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA9340.19311325865184614No Hit
CTCCGAAAGAGACCAAATATTCAATTACAGTCTCTCTTCCAAAAGTAAGG9100.18815103359012847No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9060.1873239960798422No Hit
AGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGG8860.18318880852841082No Hit
ATTGCTTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTTGCTGC8710.18008741786483726No Hit
ATTGATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG8300.1716102833844029No Hit
GTGAATGCCTGTTTAGCTTGTATACAGGCATACAAAGGCATTAAGGCAGG8050.16644129894511367No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT8040.16623453956754208No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG7850.16230611139368226No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC7820.16168583326096755No Hit
CCTACTCTCCTCCCCCAGCTCCTCCCAGTCTTTAAACACACAGTCTTTGA7230.14948702998424493No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA7210.1490735112291018No Hit
GGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAATGGAGCAG7170.1482464737188155No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTTTTGGT7120.14721267683095765No Hit
AGATAAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGG7100.14679915807581453No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG6980.14431804554495567No Hit
CCCTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTG6950.143697767412241No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6880.14225045176924No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT6720.13894230172809488No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA6690.13832202359538018No Hit
CAATTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACAAT6640.13728822670752233No Hit
ATTTCCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAAACAAAAAGA6460.13356655791123406No Hit
GGCTAAATGTATGCTAATGTAAGTGTTCTGAGCACATTTAAGGTAGGCTA6440.13315303915609092No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA6330.13087868600280367No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATCAGAGGACAAACGGGCAAC6320.13067192662523208No Hit
CATTTAAGGTAGGCTAAGCTAAGCTATGAGGTCCAGTAGGTTAGGTGTAT6150.1271570172065154No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT6130.12674349845137228No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAA6110.1263299796962291No Hit
GTGCTGGTGGGAGAGTAGCAGTGAGGACGACCAGAGTCACTCTCATCGCA6090.12591646094108597No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGACGATGGGATGGGAATACAGGTG6060.12529618280837126No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC6000.12405562654294186No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT5960.12322858903265559No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5930.12260831089994087No Hit
CCTATCAATAATGTCCTCTTGTAAATGAATTTTAGGAAAGGAAGGAGTTT5880.12157451401208301No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCCTGTAT5800.11992043899151048No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC5800.11992043899151048No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5780.11950692023636732No Hit
GTGTTACATGATTTGGCCCAAACATAGGCTAAATGTATGCTAATGTAAGT5750.11888664210365261No Hit
ATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGCACCTG5730.11847312334850948No Hit
CCAATAGTCCTCTTATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCAC5630.11640552957279378No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC5570.11516497330736435No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT5540.11454469517464966No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA5500.11371765766436336No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5480.11330413890922023No Hit
CCCTAAACTTCATGGGTTACATAATTGGAAGTTGGGGAACATTGCCACAG5420.11206358264379082No Hit
CTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAA5380.11123654513350452No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA5380.11123654513350452No Hit
ATTTAAGATGGAGTTGCTCTGGTTCACTTGTCTCTGACAAGTTGAAAATG5370.11102978575593296No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5370.11102978575593296No Hit
GGCCTTCACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAACAGTACA5370.11102978575593296No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAATTA5340.11040950762321824No Hit
GTCTTTATGATCTGTATTTTGTGCTGACCTCCTATCTCATTCTATGACTT5310.10978922949050353No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGATCACCCGTG5240.10834191384750255No Hit
GTCTAAGGGCAAATATTTAGTTTTGGTAGGATAGAATCTAGCAGGCATAA5190.10730811695964469No Hit
CTCTAAGGCTTCTCGATACAGAGCTGAGGCGGTGTCTAGGAGATCTCTGA5190.10730811695964469No Hit
GCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGCGCCCC5190.10730811695964469No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA5170.10689459820450156No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT5120.1058608013166437No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT5100.10544728256150057No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5080.10503376380635743No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAAATTCATTTACAAG5040.10420672629607117No Hit
GTTTTGTTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTT5030.10399996691849958No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT5020.10379320754092802No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCCTGTATACAAGCTAAACAG4970.10275941065307018No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC4970.10275941065307018No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT4910.10151885438764074No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT4890.1011053356324976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)