FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479805
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA20050.4178780963099592No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18380.3830722897843916No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT15510.32325632288117045No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT15050.3136690947364033No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC13600.28344848428007213No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC10260.21383687122893674No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10060.20966851116599453No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10010.20862642115025898No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT10010.20862642115025898No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA9590.19987286501808027No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT8750.18236575275372288No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT8410.17527954064672108No Hit
GTCCAAGAGTCCTCTTATGTAAGACAGAGAGTCCAAGAGTCCTCTTATGT8380.17465428663727972No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT8180.1704859265743375No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA8150.16986067256489615No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACTCCTCCC7750.1615239524390117No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7540.15714717437292233No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7430.1548545763383041No Hit
GCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG7430.1548545763383041No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC7280.15172830629109743No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG7260.1513114702848032No Hit
CACTAATATGGGCTTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCTT7200.15006096226592053No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6750.14068215212430052No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6660.13880639009597648No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6620.13797271808338804No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACAGGG6470.13484644803618137No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6360.13255385000156314No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC6160.1283854899386209No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA6110.12734339992288535No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6110.12734339992288535No Hit
ATCTAGAAGATGTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6030.12567605589770844No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG5990.12484238388511999No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5960.12421712987567866No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5960.12421712987567866No Hit
CTTACAGTCAATGAAAAACGAAGACTGCAATTAATTATGCCTGCTAGGTT5890.12275820385364887No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC5880.12254978585050176No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5870.12234136784735465No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5800.12088244182532487No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACT5800.12088244182532487No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTT5780.12046560581903064No Hit
TTGACAGACTTTCCAATCAATTGGCCTGTTAACAGGAAGTTTTCTAAAAC5780.12046560581903064No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5740.11963193380644221No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5730.11942351580329509No Hit
CTTTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA5720.11921509780014798No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA5700.11879826179385375No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5650.11775617177811819No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5580.11629724575608841No Hit
CTTATATAAGATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5560.11588040974979419No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5530.11525515574035285No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGAT5510.11483831973405864No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5450.11358781171517596No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5450.11358781171517596No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5410.11275413970258752No Hit
ATATTAGTGTTGACATAACTAACAACTAGGTCCCTAGATGCTGGATCTTC5320.11087837767426349No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5320.11087837767426349No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5240.1092110336490866No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5190.10816894363335104No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACAGGG5150.1073352716207626No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5130.10691843561446837No Hit
GACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTTAG5110.10650159960817415No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5050.1052510915892915No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT5020.10462583757985015No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4990.10400058357040881No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4980.10379216556726169No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT4970.10358374756411458No Hit
CTCTTATATAAGATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT4920.10254165754837903No Hit
CTTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGA4920.10254165754837903No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4900.1021248215420848No Hit
GGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAA4890.10191640353893769No Hit
CTCTTGGACTCTCTGTCTTACATAAGAGGACTCTTGGACTCTCTGTAATG4840.10087431352320214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)