FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510182
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26090.5113861327918272No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG20900.4096577299865538No Hit
ATATTGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGTCCCTTTATACC18290.3584995158590464No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA16070.314985632578178No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT15690.3075373102147861No Hit
ATACAATATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA15480.3034211320665958No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13380.2622593505846933No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA12770.25030283310661683No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11730.22991795084891273No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11730.22991795084891273No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA11640.22815387449968833No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGG11480.22501773876773384No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA11400.22344967090175663No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT11140.2183534503373306No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC10720.21012109404095008No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT10360.20306478864405253No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT10220.20032066987859237No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT10130.19855659352936797No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG9690.18993222026649315No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT9620.18856016088376304No Hit
ACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATT9080.17797570278841668No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA9030.17699566037218092No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC9010.1766036434056866No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG8560.16778326165956461No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA8470.1660191853103402No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC8360.1638630919946215No Hit
GTACCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG8360.1638630919946215No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG8300.1626870410951386No Hit
GGTCTGGAAGTAATGATTAACTACCTGATCTGGATAATAAGGTTTAATTC8170.16013893081292557No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA8170.16013893081292557No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT7940.155630735698241No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGG7770.1522985914830394No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA7670.15033850665056783No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA7620.14935846423433205No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATATTTAACCT7490.14681035395211905No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT7380.14465426063640033No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGTCCCTTTATACC7270.14249816732068163No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC7080.1387740061389857No Hit
GAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTATTTTGCGAAAG7040.13798997220599707No Hit
CTCTTATATAGAACACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT6670.13073765832585232No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC6430.12603345472792063No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG6400.12544542927817914No Hit
ATGTAAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGATCTG6320.12387736141220192No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT6270.12289731899596615No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT6250.12250530202947184No Hit
CTTATATAGAACACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT6250.12250530202947184No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6230.12211328506297751No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT6190.12132925112998891No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG6170.1209372341634946No Hit
GTATCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA6100.1195651747807645No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT6040.11838912388128157No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA6040.11838912388128157No Hit
GTTTCGCTTGTATACATGCATACAAAGGCATTAAGGCAGGATATCCACAT5990.1174090814650458No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG5840.11446895421633849No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5730.11231286090061979No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT5700.11172483545087832No Hit
GATCTGGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTG5670.11113681000113684No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGTGGGTCACCATA5610.10996075910165391No Hit
GTACTTGAATATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5570.1091767251686653No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG5490.10760865730268807No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC5460.10702063185294661No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC5390.1056485724702165No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT5340.10466853005398073No Hit
GTTCAGGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5330.10447252157073357No Hit
GCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTT5210.10212041977176772No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5190.10172840280527343No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA5150.10094436887228479No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA5150.10094436887228479No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)