FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510182
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26070.5109941158253329No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG19570.38358860171468223No Hit
ATATTGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGTCCCTTTATACC18580.3641837618732139No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14730.2887204958230592No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT13980.27401985957952263No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA13760.26970767294808523No Hit
ATACAATATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA13620.266963554182625No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA12550.24599064647517943No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11980.2348181629300916No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA11310.22168559455253223No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT10810.2118851703901745No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA10570.2071809667922428No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGG10300.20188873774456959No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT10300.20188873774456959No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG10140.1987526020126151No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT10080.19757655111313216No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT10000.19600848324715495No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC9880.19365638144818909No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9680.18973621178324598No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT9280.18189587245335978No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC9280.18189587245335978No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG9160.17954377065439392No Hit
GTACCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG9040.17719166885542806No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA8750.17150742284126058No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA8260.16190300716214998No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA8200.16072695626266703No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC8130.15935489687993698No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG7940.155630735698241No Hit
ACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATT7830.1534746423825223No Hit
GGTCTGGAAGTAATGATTAACTACCTGATCTGGATAATAAGGTTTAATTC7620.14935846423433205No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA7560.14818241333484913No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGG7470.14641833698562473No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT7120.1395580400719743No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC7020.13759795523950274No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT6990.13700992978976131No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGTCCCTTTATACC6860.1344618195075483No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATATTTAACCT6840.13406980254105397No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6620.12975761590961657No Hit
GTTTCGCTTGTATACATGCATACAAAGGCATTAAGGCAGGATATCCACAT6560.12858156501013362No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6520.12779753107714503No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG6440.1262294632111678No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC6340.12426937837869623No Hit
CTCTTATATAGAACACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT6300.1234853444457076No Hit
GTATCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA6280.12309332747921331No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT6280.12309332747921331No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC6280.12309332747921331No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG6270.12289731899596615No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG6190.12132925112998891No Hit
GAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTATTTTGCGAAAG6150.12054521719700027No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA5990.1174090814650458No Hit
GTACTTGAATATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5960.11682105601530433No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT5530.10839269123567667No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG5510.10800067426918238No Hit
CTTATATAGAACACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT5500.10780466578593521No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT5410.10604058943671082No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT5410.10604058943671082No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT5400.10584458095346366No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT5350.1048645385372279No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGTGGGTCACCATA5330.10447252157073357No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA5220.10231642825501489No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTTCGCGGGACGTCCTTTGTTTA5190.10172840280527343No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA5160.10114037735553195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)