FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563303
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46260.8212276519031497No Hit
TCCATAGAGTGTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGA41080.7292700376174102No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16060.2851041091561735No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC15860.2815536221181141No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG15530.275695318505316No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT13370.23735005849427393No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC11360.20166766376177653No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG11200.19882727413132895No Hit
GGATAAGGGTATTAAACCTTACTATCCAGATCATCTAGTTAATCATTACT11080.1966969819084933No Hit
GGATAGTAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT10400.18462532597909118No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTAGTTT10110.179477119773905No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9770.17344129180920392No Hit
GTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGATGATCTGGAT9230.16385497680644343No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG9200.1633224037507345No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8980.15941686800886912No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATTATTAATCT8630.1532035156922651No Hit
GATCTGGATAGTAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA8550.15178332087704133No Hit
GTATAATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC8480.1505406504137205No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTAGT8400.1491204555984967No Hit
GTATTAAACCTTACTATCCAGATCATCTAGTTAATCATTACTTCCAAACT8270.1468126390237581No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG8190.1453924442085343No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8150.1446823468009224No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8100.14379472504140756No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8080.14343967633760163No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAC7990.14184195717047487No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC7830.13900156754002732No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTAGTTTGGA7810.13864651883622137No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7740.13740384837290057No Hit
CTCACAGTCAATGAAAAAAGAAGACTGCAATTAATTATGCCTGCTAGGTT7510.13332078827913219No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7070.1255097167954014No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC7010.12444457068398358No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT6890.12231427846114791No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6820.12107160799782711No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACA6800.12071655929402116No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAC6380.11326053651409632No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTAGTTTGGAAGT6360.11290548781029038No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6290.11166281734696958No Hit
GTGTGAAAGGGGCGGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6160.10935500077223093No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6150.10917747642032796No Hit
TCTCCTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTT6110.10846737901271607No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6000.10651461114178339No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTGAAA5790.10278659975182095No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5720.10154392928850016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)