FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610699
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG49770.8149677664446806No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGG38130.6243665046119282No Hit
CTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT35420.5799911249240625No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG29130.4769943949474291No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT22130.36237164298615193No Hit
CTCTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT19610.32110745228009213No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT18430.30178533123519113No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAATCTCCTTTTTTCA17010.2785332872659035No Hit
TTATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA16890.27656832580371016No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA16790.27493085791854904No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT16120.26395982308796967No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA14370.2353041350976504No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14150.23170170575029597No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTT13850.22678930209481266No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13380.21909320303455546No Hit
ATATGTTCCTGTGGCAAAGAACCCCAACTTCCAATTACATATCCCATGAA13250.21696449478384605No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT12890.21106961039726607No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC12570.20582971316475054No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA12080.19780612052746116No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTTCTTTGCCACAG11690.19141999577533286No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT11450.1874900728509462No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11240.1840513902921079No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTGAAATTAAT11070.18126769488733402No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA11020.1804489609447535No Hit
CTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCTCTTTTCCTAAC10890.17832025269404403No Hit
GTATACACGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10800.17684653159739905No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA10340.16931417932565798No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC10260.16800420501752908No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCTCTTTTC10220.16734921786346466No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC10190.1668579774979163No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG9870.1616180802654008No Hit
CTGTAAGAGGGCCCACATATTGTTGACACCTATTAATAATGTCCTCCTGT9750.15965311880320748No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9730.15932562522617524No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9640.15785190412953026No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC9570.1567056766099175No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9530.15605068945585304No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC9510.15572319587882083No Hit
GTACAATATGTTCCTGTGGCAAAGAACCCCAACTTCCAATTACATATCCC9490.15539570230178862No Hit
CTCTGACAGTTAATGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGG9420.15424947478217585No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAACGTAAGA9420.15424947478217585No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG9040.14802709681856363No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9020.14769960324153142No Hit
TCCCGATACAGAGCAGATGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA8920.14606213535637033No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8900.1457346417793381No Hit
ACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA8850.14491590783675753No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8800.144097173894177No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG8770.1436059335286287No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC8710.14262345279753202No Hit
GGATAAAACCTAGCAGGCATAATTAATTTCAATCTCCTTTTTTCATTAAC8550.14000350418127425No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8420.13787479593056481No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8320.1362373280454037No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG8240.13492735373727482No Hit
CTCTAAGGCCTCCCGATACAGAGCAGATGCGGTGTCGAGGAGATCTCGAA8210.1344361133717265No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC8090.13247115190953318No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT8030.13148867117843652No Hit
CCTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTT7960.13034244365882375No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7830.12821373540811432No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7810.12788624183108208No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7800.12772249504256597No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7790.1275587482540499No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA7630.1249387996377921No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCGTGTATA7570.12395631890669544No Hit
CTTACAGTTAATGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGGTT7390.12100887671340546No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTTG7350.12035388955934101No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC7310.11969890240527657No Hit
ATCTCAATCGCCGCGTCGCAGAAGATCTCAATCTCGGGAATCTCAATGTT7290.11937140882824436No Hit
GGAACATATTGTACTAAAACTTAAGCAATGTTTTCGGAAACTGCCTGTAA7270.11904391525121213No Hit
ACACAAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA7260.11888016846269603No Hit
CTTTATATGCGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACT7230.1183889280971477No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7180.11757019415456714No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7160.11724270057753491No Hit
CTTTAACCCTGAGTGGCAAACTCCCTCTTTTCCTAACATTCATTTACAGG7150.11707895378901881No Hit
CACATATTGTTGACACCTATTAATAATGTCCTCCTGTAAATGAATGTTAG7090.11609647305792214No Hit
ATTTACATACTCTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACA7020.11495024553830938No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7020.11495024553830938No Hit
ATTGACAACAGTGCCCGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6970.11413151159572882No Hit
GTCTTTAACCCTGAGTGGCAAACTCCCTCTTTTCCTAACATTCATTTACA6970.11413151159572882No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAA6960.11396776480721271No Hit
CGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6940.11364027123018049No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6920.11331277765314829No Hit
ATACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAG6900.11298528407611605No Hit
TCCTAGTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAGT6880.11265779049908382No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6870.11249404371056772No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTAAGTTTTAGTACAATA6780.11102032261392272No Hit
CAATTTGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTGAAATTAAT6660.1090553611517294No Hit
AATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCCTTGG6650.1088916143632133No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6620.10840037399766497No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6600.10807288042063276No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6570.10758164005508443No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6530.10692665290101998No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6510.10659915932398777No Hit
GATCTCAATCGCCGCGTCGCAGAAGATCTCAATCTCGGGAATCTCAATGT6480.10610791895843943No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6480.10610791895843943No Hit
CCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCAT6470.10594417216992331No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC6420.10512543822734277No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6280.10283298318811722No Hit
CCTTTACCCCGTTGCACGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6230.10201424924553668No Hit
ACCAAGCTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6220.10185050245702056No Hit
CTTAATTTTTAGTACAATATGTTCCTGTGGCAAAGAACCCCAACTTCCAA6180.10119551530295612No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCT6180.10119551530295612No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTAAGTTTTAGTACAATATGTT6180.10119551530295612No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG6130.10037678136037557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)