FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610699
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG57450.9407253000250533No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGG36240.5934183615823835No Hit
CTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT33230.5441305782390343No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG26830.43933263358872376No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT20630.33780962470873543No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT20340.3330609678417682No Hit
CTCTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT19050.31193763212319No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAATCTCCTTTTTTCA16850.27591333864964573No Hit
TTATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA16460.26952721389751744No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA15680.25675496439326084No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14530.23792408371390816No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC14110.23104671859623155No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTT14080.23055547823068323No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13690.2241693534785549No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTTCTTTGCCACAG13270.21729198836087826No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13200.21614576084126552No Hit
ATATGTTCCTGTGGCAAAGAACCCCAACTTCCAATTACATATCCCATGAA12910.21139710397429828No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT12890.21106961039726607No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA12440.2037010049140411No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA12380.20271852418294445No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC12180.19944358841262225No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT11060.1811039480988179No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA10920.17881149305959237No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA10750.1760277976548185No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA10550.1727528618844963No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTGAAATTAAT10270.1681679518060452No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAACGTAAGA10180.1666942307094002No Hit
GTATACACGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10120.16571174997830354No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC10020.16407428209314245No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCTCTTTTC9850.16129058668836857No Hit
GGATAAAACCTAGCAGGCATAATTAATTTCAATCTCCTTTTTTCATTAAC9650.15801565091804637No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9610.15736066376398194No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9560.1565419298214014No Hit
CTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCTCTTTTCCTAAC9490.15539570230178862No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9410.15408572799365972No Hit
CTGTAAGAGGGCCCACATATTGTTGACACCTATTAATAATGTCCTCCTGT9340.15293950047404695No Hit
GTACAATATGTTCCTGTGGCAAAGAACCCCAACTTCCAATTACATATCCC9250.15146577937740197No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC9060.14835459039559587No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG9060.14835459039559587No Hit
CTCTGACAGTTAATGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGG8840.14475216104824143No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG8710.14262345279753202No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC8620.14114973170088702No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8580.1404947445468226No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT8430.13803854271908092No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8290.13574608767985538No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG8250.13509110052579093No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8200.1342723665832104No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG8190.13410861979469427No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8160.13361737942914595No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8010.1311611776014043No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC8010.1311611776014043No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7940.1300149500817915No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG7920.1296874565047593No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA7910.1295237097162432No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCGTGTATA7760.12706750788850155No Hit
CTCTAAGGCCTCCCGATACAGAGCAGATGCGGTGTCGAGGAGATCTCGAA7680.12575753358037264No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7640.12510254642630822No Hit
TCCCGATACAGAGCAGATGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA7550.12362882532966323No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTTG7490.12264634459856656No Hit
CTTACAGTTAATGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGGTT7480.12248259781005046No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTAAGTTTTAGTACAATA7450.12199135744450212No Hit
CTTTATATGCGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACT7420.12150011707895379No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7350.12035388955934101No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC7310.11969890240527657No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7200.11789768773159937No Hit
ATTTACATACTCTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACA7130.11675146021198658No Hit
GGAACATATTGTACTAAAACTTAAGCAATGTTTTCGGAAACTGCCTGTAA7110.11642396663495437No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC7040.11527773911534162No Hit
CCTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTT7040.11527773911534162No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7020.11495024553830938No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7020.11495024553830938No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT7010.11478649874979328No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAA7000.11462275196127715No Hit
ATCTCAATCGCCGCGTCGCAGAAGATCTCAATCTCGGGAATCTCAATGTT6940.11364027123018049No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6850.11216655013353552No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6820.11167530976798719No Hit
CCTTTACCCCGTTGCACGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6750.11052908224837439No Hit
CTTTAACCCTGAGTGGCAAACTCCCTCTTTTCCTAACATTCATTTACAGG6720.11003784188282606No Hit
ACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA6720.11003784188282606No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6610.10823662720914887No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA6430.10528918501585888No Hit
CGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6420.10512543822734277No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6420.10512543822734277No Hit
ATTGACAACAGTGCCCGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6410.10496169143882665No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6330.10365171713069778No Hit
CACATATTGTTGACACCTATTAATAATGTCCTCCTGTAAATGAATGTTAG6310.10332422355366555No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6300.10316047676514944No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6230.10201424924553668No Hit
ATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAG6190.10135926209147222No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCT6190.10135926209147222No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6130.10037678136037557No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6110.10004928778334335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)